infestation in wheat is highly susceptible to common root rot and leaf black spot diseases, leading to significant yield loss. The detrimental effects of chemical fungicides are evident. However, the development of new biological control methods that meet the requirements of environmentally friendly and sustainable agriculture is still underway. In this study, we screened and identified a bacterial strain, JK-1, which exhibited significant antagonistic effects against , as In the present study, the fermentation filtrate of the antagonist strain JK-1 was prepared and its inhibitory effect on was investigated. Treatment with 20% JK-1 culture filtrate (CF) resulted in a reduction of 65.8% in the dry weight of mycelium and a decrease of 93.3% in the spore germination rate. Scanning electron microscopy (SEM) and laser scanning confocal microscopy (LSCM) revealed that the CF of JK-1 caused significant damage to the integrity of the cell membrane of Additionally, LSCM demonstrated that CF treatment led to increased DNA leakage and the accumulation of reactive oxygen species (ROS) in mycelial cells. Moreover, the disruption of the antioxidant defense system of by CF was demonstrated through the assessment of key antioxidant enzyme activities. The crude extract of the JK-1 CF was analyzed using liquid chromatography time-of-flight mass spectrometry (LC-TOF-MS) and was determined to contain the lipopeptide surfactin. JK-1 exhibited significant control effects in biocontrol experiments involving detached leaves and potting. Furthermore, the JK-1 CF was found to significantly promote the growth of wheat seedlings. These results indicate that JK-1 holds great potential as a strain for controlling wheat root rot and can provide a new approach to wheat management.

中文翻译：

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Bacillus valezensis 菌株 JK-1 作为小麦根腐病的有效生物防治策略

小麦的侵染极易发生常见的根腐病和叶黑斑病，导致产量严重损失。化学杀菌剂的有害影响是显而易见的。然而，满足环境友好和可持续农业要求的新生物防治方法的开发仍在进行中。本研究筛选鉴定了一株对 β-内毒素具有显着拮抗作用的菌株 JK-1，本研究制备了拮抗菌株 JK-1 的发酵滤液，并考察了其抑制作用。用20％JK-1培养滤液(CF)处理导致菌丝干重减少65.8％，孢子萌发率降低93.3％。扫描电子显微镜（SEM）和激光扫描共聚焦显微镜（LSCM）显示，JK-1的CF对细胞膜的完整性造成了显着损害。此外，LSCM表明CF处理导致DNA泄漏增加和反应性物质的积累。菌丝细胞中的氧物质（ROS）。此外，通过评估关键抗氧化酶活性，证明了 CF 对抗氧化防御系统的破坏。使用液相色谱-飞行时间质谱 (LC-TOF-MS) 分析 JK-1 CF 的粗提物，并确定其含有脂肽表面活性素。 JK-1在涉及落叶和盆栽的生物防治实验中表现出显着的防治效果。此外，JK-1 CF被发现能显着促进小麦幼苗的生长。这些结果表明JK-1作为控制小麦根腐病的菌株具有巨大的潜力，可以为小麦管理提供新的途径。