Medicinal plant extracts include a multitude of chemical compounds. Therefore, there is a need for suitable analytical methods for their identification and standardization. The aim of the present study was to extract, isolate, and identify rutin from Capparis zeylanica (CZ) leaves and determine its content in the stems, leaves, roots, and flowers of CZ plant by a simple, sensitive, precise, accurate, and specific high-performance thin-layer chromatography (HPTLC) method for the first time. The HPTLC method used silica gel 60 F₂₅₄ for the stationary phase, ethyl acetate‒glacial acetic acid‒formic acid–water (10:1.1:1.1:2.6, V/V) as the mobile phase, and scanning under ultraviolet (UV) 264 nm light, by using deuterium lamp, without chemical reagents. The calibration curves for rutin were found to be linear in the range between 400 and 1400 ng per spot with R² = 0.9953 ± 0.0004. This method showed good peak symmetry for rutin (R_F: 0.418 ± 0.004) with limit of detection and limit of quantification of 14.10 and 42.73 ng/spot, respectively. The precision, accuracy, robustness, and specificity studies for CZ extracts and rutin are within the limits as per the International Council for Harmonisation guidelines. This study confirms the presence of rutin with content (% w/w) of 3.16 ± 0.01 in leaves, 5.92 ± 0.04 in stems, and 2.23 ± 0.02 in flowers, on dry weight basis of CZ extracts. However, it is not found at detectable levels in the roots of CZ plant. In conclusion, the proposed method is simple, reliable, and specific for the quantification of rutin. The results also show that CZ could be an important nutritional source of rutin.

药用植物提取物包含多种化合物。因此，需要合适的分析方法来对其进行鉴定和标准化。本研究的目的是从Capparis zeylanica (CZ)叶中提取、分离和鉴定芦丁，并通过一种简单、灵敏、精密、准确的方法测定其在CZ植物的茎、叶、根和花中的含量。首次采用特定的高效薄层色谱（HPTLC）方法。 HPTLC方法以硅胶60 F ₂₅₄为固定相，乙酸乙酯-冰醋酸-甲酸-水（10:1.1:1.1:2.6，V/ V）为流动相，紫外（UV）下扫描264 nm 光，使用氘灯，无需化学试剂。发现芦丁的校准曲线在每点 400 至 1400 ng 范围内呈线性，R ²  = 0.9953 ± 0.0004。该方法显示芦丁具有良好的峰对称性（RF ：0.418 ± 0.004） _，检测限和定量限分别为 14.10 和 42.73 ng/点。 CZ 提取物和芦丁的精密度、准确度、稳健性和特异性研究均在国际协调委员会指南的限制范围内。本研究证实，以 CZ 提取物的干重计，芦丁的含量（% w / w ）在叶子中为 3.16 ± 0.01，在茎中为 5.92 ± 0.04，在花中为 2.23 ± 0.02。然而，在 CZ 植物的根部中未发现可检测到的水平。综上所述，该方法简单、可靠、专属性强，适用于芦丁的定量分析。结果还表明，CZ 可能是芦丁的重要营养来源。