Purpose

The purpose of this study was to investigate the role of lncRNA DSCAM-AS1 in prostate cancer to find new therapeutic targets and promote the research progress of prostate cancer.

Methods

RT-qPCR was used to detect DSCAM-AS1 expression in prostate cancer tissues, normal tissues, human normal prostate epithelial cells (RWPE), and four prostate cancer cell lines. The clinical and prognostic role of DSCAM-AS1 was evaluated by the Kaplan–Meier curve and chi-square test. Secondly, a dual luciferase reporter gene assay was used to study the regulatory mechanism between miR-338-3p and DSCAM-AS1. Finally, the roles of DSCAM-AS1 and miR-338-3p in prostate cancer cell proliferation and metastasis were explored by CCK-8 and Transwell assays.

Results

It was found that DSCAM-AS1 upregulation could serve as a warning of deterioration and poor prognosis in prostate cancer patients, and that knockdown of DSCAM-AS1 expression inhibited the progression of prostate cancer cells. In addition, miR-338-3p, a target of DSCAM-AS1, was found to be down-regulated in prostate cancer cells and miR-338-3p knockdown could reverse the inhibitory effect of DSCAM-AS1 silencing on prostate cancer.

Conclusion

DSCAM-AS1 is up-regulated in prostate cancer and regulates the progression of prostate cancer cells by targeting miR-338-3p.

中文翻译：

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DSCAM-AS1促进前列腺癌的发展

目的

本研究旨在探讨lncRNA DSCAM-AS1在前列腺癌中的作用，寻找新的治疗靶点，推动前列腺癌的研究进展。

方法

采用RT-qPCR检测前列腺癌组织、正常组织、人正常前列腺上皮细胞（RWPE）和四种前列腺癌细胞系中DSCAM-AS1的表达。 DSCAM-AS1 的临床和预后作用通过 Kaplan-Meier 曲线和卡方检验进行评估。其次，采用双荧光素酶报告基因检测研究miR-338-3p和DSCAM-AS1之间的调控机制。最后，通过CCK-8和Transwell实验探讨了DSCAM-AS1和miR-338-3p在前列腺癌细胞增殖和转移中的作用。

结果

研究发现，DSCAM-AS1 上调可以作为前列腺癌患者病情恶化和预后不良的警告，而 DSCAM-AS1 表达的敲低可以抑制前列腺癌细胞的进展。此外，发现 DSCAM-AS1 的靶标 miR-338-3p 在前列腺癌细胞中下调，并且 miR-338-3p 敲低可以逆转 DSCAM-AS1 沉默对前列腺癌的抑制作用。

结论

DSCAM-AS1 在前列腺癌中上调，并通过靶向 miR-338-3p 来调节前列腺癌细胞的进展。