20‐Hydroxyecdysone (20E) plays a vital role in a series of biological processes, via the nuclear receptors, EcR/USP by activating the ecdysone regulatory cascade. To clarify the role of EcR during the development of Grapholita molesta, the complementary DNA of ecdysone receptor isoform B1 (GmEcR‐B1) was obtained from the transcriptome of G. molesta and verified by PCR. Alignment analysis revealed that the deduced protein sequence of GmEcR‐B1 was highly homologous to EcR proteins identified in other lepidopteran species, especially the EcR‐B1 isoform in Spodoptera litura. Quantitative real‐time PCR showed that GmEcRs was expressed at all test developmental stages, and the expression level of GmEcRs was relatively higher during the period of the 3rd day of fifth instar larvae to 2nd of pupa than those in other stages. Moreover, the messenger RNA of GmEcRs was much more strongly expressed in the Malpighian tubule and epidermis than those in other tissues, which suggests that this gene may function in a tissue‐specific manner during larval development. Silencing of GmEcRs could significantly downregulate the transcriptional level of ecdysone‐inducible genes and result in increased mortality during metamorphosis and prolonged prepupal duration. Taken together, the present results indicate that GmEcRs may directly or indirectly affect the development of G. molesta.

中文翻译：

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东方果蛾 Grapholita molecularsta（鳞翅目：Tortricidae）蜕皮激素受体亚型 (EcR) 的分子表征和功能分析

20-羟基蜕皮激素 (20E) 通过核受体、EcR/USP 激活蜕皮激素调节级联，在一系列生物过程中发挥着至关重要的作用。阐明 EcR 在开发过程中的作用梨小食心虫，蜕皮激素受体亚型 B1 的互补 DNA（GmEcR-B1）是从转录组中获得的摩尔斯塔并通过PCR验证。比对分析表明，推导的 GmEcR-B1 蛋白序列与其他鳞翅目物种中鉴定的 EcR 蛋白高度同源，特别是斜纹夜蛾。实时定量 PCR 表明GmEcR在所有测试发育阶段都有表达，并且表达水平GmEcR五龄幼虫第3天至第2蛹期间相对较高。此外，信使RNAGmEcR与其他组织相比，该基因在马氏小管和表皮中的表达更加强烈，这表明该基因在幼虫发育过程中可能以组织特异性方式发挥作用。沉默GmEcR可以显着下调蜕皮激素诱导基因的转录水平，导致变态期间死亡率增加和预蛹持续时间延长。综上所述，目前的结果表明GmEcR可能会直接或间接影响事物的发展摩尔斯塔。