[FeFe]-hydrogenase (HydA) is an active biocatalytic enzyme for solar-to-hydrogen (H₂) conversion. However, stability is a main challenge that limits practical applications. This work aims to encourage the efficiency of HydA by encapsulating it in the zeolitic imidazolate framework-8 (ZIF-8) as a synthetic protective shield. The construction of HydA@ZIF-8 nanoparticles, with an average diameter of 700–1000 nm, at ambient conditions can preserve HydA activity within a spatially confined microenvironment, as characterized by scanning electron microscopy and X-ray diffraction analysis. Based on MV^•+-dependent H₂ production activity and kinetic analysis, both the stability and efficiency of HydA@ZIF-8 surpass those of free HydA and whole-cell biocatalysts over a wider range of pH and temperature. The achievement of robust HydA@ZIF-8 construction represents a significant step forward in the development of biocatalysts for various future applications.

中文翻译：

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封装在沸石咪唑酯框架 (ZIF)-8 纳米颗粒中的 [FeFe]-氢化酶作为光催化制氢的稳健生物催化剂

[FeFe]-氢化酶 (HydA) 是一种用于将太阳能转化为氢气 (H ₂ ) 的活性生物催化酶。然而，稳定性是限制实际应用的主要挑战。这项工作旨在通过将 HydA 封装在沸石咪唑骨架 8 (ZIF-8) 中作为合成保护罩来提高 HydA 的效率。通过扫描电子显微镜和 X 射线衍射分析，在环境条件下构建平均直径为 700-1000 nm 的 HydA@ZIF-8 纳米粒子，可以在空间有限的微环境中保留 HydA 活性。基于MV ^•+依赖性H ₂产生活性和动力学分析，HydA@ZIF-8在更宽的pH和温度范围内的稳定性和效率均优于游离HydA和全细胞生物催化剂。坚固的 HydA@ZIF-8 结构的实现代表着未来各种应用的生物催化剂的开发向前迈出了重要一步。