Abstract

Glycosidic osmolytes are widespread natural compounds that protect microorganisms and their macromolecules from the deleterious effects of various environmental stresses. Their protective properties have attracted considerable interest for industrial applications, especially as active ingredients in cosmetics and healthcare products. In that regard, the osmolyte glucosylglycerate is somewhat overlooked. Glucosylglycerate is typically accumulated by certain organisms when they are exposed to high salinity and nitrogen starvation, and its potent stabilizing effects have been demonstrated in vitro. However, the applications of this osmolyte have not been thoroughly explored due to the lack of a cost-efficient production process. Here, we present an overview of the progress that has been made in developing promising strategies for the synthesis of glucosylglycerate and its precursor glycerate, and discuss the remaining challenges.

Key points

• Bacterial milking could be explored for fermentative production of glucosylglycerate

• Glycoside phosphorylases of GH13_18 represent attractive alternatives for biocatalytic production

• Conversion of glycerol with alditol oxidase is a promising strategy for generating the precursor glycerate

中文翻译：

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渗透剂葡萄糖基甘油酸酯及其前体甘油酸酯的合成策略

摘要

糖苷渗透剂是广泛存在的天然化合物，可保护微生物及其大分子免受各种环境压力的有害影响。它们的保护特性引起了工业应用的极大兴趣，特别是作为化妆品和保健产品中的活性成分。在这方面，渗透剂葡萄糖基甘油酸酯在某种程度上被忽视了。某些生物体在暴露于高盐度和氮饥饿时通常会积累葡萄糖基甘油酸，其有效的稳定作用已在体外得到证明。然而，由于缺乏具有成本效益的生产工艺，这种渗透剂的应用尚未得到彻底探索。在这里，我们概述了在开发有前景的葡萄糖基甘油酸酯及其前体甘油酸酯合成策略方面所取得的进展，并讨论了仍然存在的挑战。

关键点

•可以探索细菌挤奶用于葡萄糖基甘油酸的发酵生产

• GH13_18 糖苷磷酸化酶是生物催化生产的有吸引力的替代品

•用糖醇氧化酶转化甘油是产生前体甘油酸的一种有前景的策略