Fluorescence‐detected linear dichroism (FD‐LD) enables one to collect linear dichroism spectra for oriented fluorophores in the presence of other absorbing species and light scattering. The experiment proceeds by scanning the excitation wavelength and using a filter to collect only emitted photons from the fluorophore. Thus, it has the potential to give data with enhanced selectivity and quality. By using a synchrotron radiation light source and fluorescence‐detection, we show data for a range of fluorophores in different orienting environments. Film and flow‐oriented FD‐LD spectra were collected down to 170 nm. Even for flow‐oriented liposomes, we have data collected down to 210 nm. For strongly scattering samples, for example, liposomes, FD‐LD has the clear advantage that scattering is absent for the longer wavelength fluorescence photons. The collimated and smaller beam size of the synchrotron radiation also gives rise to sharper and more well‐defined features in the spectra.

中文翻译：

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同步加速器光源用于荧光检测线性二向色性的优点

荧光检测线性二色性 (FD-LD) 使人们能够在存在其他吸收物质和光散射的情况下收集定向荧光团的线性二色性光谱。该实验通过扫描激发波长并使用滤光片仅收集荧光团发射的光子来进行。因此，它有可能提供具有更高选择性和质量的数据。通过使用同步辐射光源和荧光检测，我们显示了不同定向环境中一系列荧光团的数据。收集了低至 170 nm 的薄膜和流向 FD-LD 光谱。即使对于流向脂质体，我们也收集了低至 210 nm 的数据。对于强散射样品，例如脂质体，FD-LD 具有明显的优势，即较长波长的荧光光子不存在散射。同步加速器辐射的准直且较小的光束尺寸也使光谱中的特征更清晰、更清晰。