BackgroundThis study evaluated the presence of Epstein-Barr virus type 1 (EBV-1) DNA in patients living with HIV, before and after three different topical therapy protocols for oral hairy leukoplakia (OHL).MethodsThe sample consisted of five patients treated with topical solution of 25% podophyllin resin; six with 25% podophyllin resin plus 5% acyclovir cream; and four with 25% podophyllin resin plus 1% penciclovir cream. DNA was extracted from OHL scrapings and amplified by the PCR using specific primers for EBV-1 (EBNA-1).ResultsClinical healing of OHL lesions was observed across all treatment groups over time. At baseline, EBNA-1 was detected in all OHL lesions. After treatment, OHL samples from three patients treated with 25% podophyllin resin plus 5% acyclovir cream and from one patient treated with 25% podophyllin resin plus 1% penciclovir cream exhibited negative EBNA-1 viral gene encoding. Despite the clinical resolution of OHL, 11 patients (73.3%) showed EBNA-1 positivity immediately after the lesion disappeared. Three patients (20%) treated with podophyllin resin displayed both EBNA-1 positivity and a recurrence of OHL, in contrast to no recurrence in the other two groups.ConclusionsThese findings suggest potential associations between treatment formulations, EBNA-1 persistence, and the recurrence of OHL lesions.

中文翻译：

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口腔毛状白斑局部治疗后 Epstein-Barr 病毒的鉴定：初步研究

背景本研究评估了 HIV 感染者在三种不同的口腔毛状白斑 (OHL) 局部治疗方案前后 EB 病毒 1 型 (EBV-1) DNA 的存在情况。方法样本由 5 名接受局部溶液治疗的患者组成25%鬼臼树脂； 25%鬼臼树脂加5%阿昔洛韦乳膏6次；四种含有 25% 鬼臼树脂加 1% 喷昔洛韦乳膏。从 OHL 刮片中提取 DNA，并使用 EBV-1 (EBNA-1) 特异性引物通过 PCR 进行扩增。结果 随着时间的推移，所有治疗组均观察到 OHL 病变的临床愈合情况。基线时，在所有 OHL 病变中均检测到 EBNA-1。治疗后，三名接受 25% 鬼臼树脂加 5% 阿昔洛韦乳膏治疗的患者和一名接受 25% 鬼臼树脂加 1% 喷昔洛韦乳膏治疗的患者的 OHL 样本显示出阴性 EBNA-1 病毒基因编码。尽管 OHL 已得到临床缓解，但仍有 11 名患者 (73.3%) 在病变消失后立即显示 EBNA-1 阳性。三名接受鬼臼树脂治疗的患者 (20%) 表现出 EBNA-1 阳性和 OHL 复发，而其他两组则没有复发。结论这些发现表明治疗配方、EBNA-1 持续性和复发之间存在潜在关联OHL 病变。