One novel compound, (R)‐3, 6‐diethoxy‐4‐hydroxycyclohex‐3‐en‐1‐one (1) and thirteen known compounds was isolated from the waste leaves of Nicotiana tabacum. The structures of three compounds (1−3) were confirmed and attributed firstly by the extensive spectroscopic data, including 1D/2D NMR, IR, HR‐ESI‐MS, CD, and ECD spectra. Notably, seven compounds (2, 3, 9, 10, 11, 12, and 13) exhibited better tyrosinase inhibitory activity than the positive control, kojic acid. The binding modes of these compounds revealed that their structure formed strong hydrogen bonds and van der Waals force with the active sites of tyrosinase. These results indicated that waste tobacco leaves are good resources for developing tyrosinase inhibitors.

中文翻译：

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废烟叶中具有酪氨酸酶抑制活性的活性成分

从烟草废叶中分离出一种新化合物 (R)-3, 6-二乙氧基-4-羟基环己-3-en-1-酮 (1) 和十三种已知化合物。三种化合物 (1−3) 的结构首先通过广泛的光谱数据得到证实和归因，包括 1D/2D NMR、IR、HR-ESI-MS、CD 和 ECD 光谱。值得注意的是，七种化合物（2、3、9、10、11、12 和 13）比阳性对照曲酸表现出更好的酪氨酸酶抑制活性。这些化合物的结合模式表明它们的结构与酪氨酸酶的活性位点形成了强氢键和范德华力。这些结果表明废弃烟叶是开发酪氨酸酶抑制剂的良好资源。