Idiopathic pulmonary fibrosis (IPF) is a chronic interstitial pneumonia with acute lung damage leading to the deterioration of lung function and increased mortality risk. In this study, we aimed to investigate the effects of the orange coproduct extract (OCE) and the combination of pure hesperidin and oleuropein (HO) on an experimental model of pulmonary fibrosis induced by bleomycin (BLM). Wistar rats were divided into 6 groups: the control group (G1), the BLM group (G2), 3 groups (G3, G4, G5) receiving a single dose of BLM combined with OCE extract at 100, 200 and 300 mg/kg and group 6 (G6) receiving a single dose of BLM combined with HO: both pure major phenolic compounds of OCE (hesperidin at 50 mg/kg) and olive leaves (oleuropein at 2.5 mg/kg). Oxidative stress in lung tissues was investigated using catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPX) assays and the measurement of malondialdehyde (MDA) and lactate dehydrogenase (LDH) levels. Treatment with OCE and HO were able to normalize the disturbance in oxidative markers' levels and showed a significant reduction in fibrosis score with no renal or hepatic toxic effects. In conclusion, OCE and HO exhibit antifibrotic effects on rat model of pulmonary fibrosis.

中文翻译：

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突尼斯橙联产品提取物和橄榄苦苷-橙皮苷组合对博来霉素诱导的大鼠肺纤维化的保护作用

特发性肺纤维化（IPF）是一种慢性间质性肺炎，伴有急性肺损伤，导致肺功能恶化并增加死亡风险。在本研究中，我们旨在研究橙联产品提取物（OCE）以及纯橙皮苷和橄榄苦苷（HO）的组合对博莱霉素（BLM）诱导的肺纤维化实验模型的影响。将 Wistar 大鼠分为 6 组：对照组（G1）、BLM 组（G2）、3 组（G3、G4、G5），接受单剂量 BLM 联合 OCE 提取物（100、200 和 300 mg/kg）第 6 组 (G6) 接受单剂量 BLM 与 H2O：两种纯主要酚类化合物 OCE（橙皮苷，50 mg/kg）和橄榄叶（橄榄苦苷，2.5 mg/kg）。使用过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPX）测定以及丙二醛（MDA）和乳酸脱氢酶（LDH）水平的测量来研究肺组织中的氧化应激。 OCE 和 H2O 治疗能够使氧化标记物水平的紊乱正常化，并显示纤维化评分显着降低，且没有肾或肝毒性作用。总之，OCE 和 HO 对大鼠肺纤维化模型具有抗纤维化作用。