Anatomical investigations aiming to seek alternative characters for the taxonomy of bamboos are almost exclusively restricted to the leaf blade. Little is known about the anatomical structure of other parts of the leaf, such as the leaf sheath and pseudopetiole. The present paper analyzed the complete structure of foliage leaves, including the foliage leaf blade, foliage leaf sheath, and pseudopetiole of species belonging to the tribes Bambuseae (subtribes Guaduinae, Chusqueinae and Arthrostylidiinae) and Olyreae (subtribe Parianinae) to identify useful characters in the taxonomy of bamboos. We used scanning electron microscopy and light microscopy to describe micromorphological and anatomical features. All species sampled share the following features: foliage leaf blades with uniseriate epidermis; adaxial bulliform cells; mesophyll with arm cells, cavities, and collateral vascular bundles. Intercostal sclerenchyma associated to bulliform cells was observed only in Arthrostylidiinae. Micromorphologically, the shape and distribution of silica bodies, presence, type, and distribution of papillae on the long cells and subsidiary cells, and the presence and distribution of trichomes may be taxonomically informative in different taxonomic levels. We highlight the absence of papillae on the surfaces of foliage leaf blade of Guaduinae since the presence of papillae is considered a putative diagnosis feature supporting the sister relationship of this subtribe with Arthrostylidiinae. We describe the anatomical structure of foliage leaf sheath for the first time for Guaduinae, Chusqueinae and Parianinae. In Guaduinae, we found a unique distribution pattern of silica cells on the abaxial surface of the leaf sheath. Anatomical data about the pseudopetiole structure also were described for the first time in bamboos species; the arrangement of vascular bundles may be taxonomically informative, especially in Guaduinae.

中文翻译：

---

新热带竹亚科（禾本科）叶片、叶鞘和假叶柄的解剖：结构和分类学贡献的见解

旨在寻找竹子分类学替代特征的解剖学研究几乎完全局限于叶片。人们对叶子其他部分（例如叶鞘和假叶柄）的解剖结构知之甚少。本文分析了属于 Bambuseae 族（Guaduinae、Chusqueinae 和 Arthrostylidiinae 亚族）和 Olyreae（Parianinae 亚族）的植物叶片的完整结构，包括叶片、叶鞘和假叶柄，以确定植物中有用的性状。竹子的分类。我们使用扫描电子显微镜和光学显微镜来描述微观形态和解剖特征。所有采样物种都具有以下特征：具有单列表皮的叶片；近轴泡状细胞；叶肉有臂细胞、空腔和侧支维管束。仅在节节虫亚科中观察到与泡状细胞相关的肋间巩膜组织。在微形态学上，硅体的形状和分布，长细胞和附属细胞上乳头的存在、类型和分布，以及毛状体的存在和分布可能在不同的分类水平上提供分类学信息。我们强调 Guaduinae 的叶子表面没有乳突，因为乳突的存在被认为是支持该亚族与节节虫亚科的姐妹关系的推定诊断特征。我们首次描述了 Guaduinae、Chusqueinae 和 Parianinae 的叶子叶鞘的解剖结构。在 Guaduinae 中，我们发现叶鞘背面的二氧化硅细胞具有独特的分布模式。关于竹类拟叶柄结构的解剖学数据也首次被描述；维管束的排列可能具有分类学信息，尤其是瓜杜亚科。