Effect-directed analysis (EDA) has been increasingly used for screening toxic contaminants in the environment, but conventional EDA procedures are often time-consuming and labor-extensive. This challenges the use of EDA for toxicant identification in the scenarios when quick answers are demanded. Herein, a solid phase microextraction ligand fishing (SPME-LF) strategy has been proposed as a rapid EDA approach for identifying acetylcholinesterase (AChE) active compounds in water. The feasibility of ligand fishing techniques for screening AChE active chemicals from environmental mixtures was first verified by a membrane separation method. Then, SPME fibers were prepared through self-assembly of boronic acid groups with AChE via co-bonding and applied for SPME-LF. As AChE coated SPME fibers selectively enriched AChE-active compounds from water, comparing sorbing compounds by the SPME fibers with and without AChE coating can quickly distinguish AChE toxicants in mixtures. Compared with conventional EDA, SPME-LF does not require repeating sample separations and bioassays, endowing SPME-LF with the merits of low-cost, labor-saving, and user-friendly. It is believed that cost-efficient and easy-to-use SPME-LF strategy can potentially be a rapid EDA method for screening receptor-specific toxicants in aquatic environment, especially applicable in time-sensitive screening.

中文翻译：

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快速筛查水中乙酰胆碱酯酶活性污染物：基于固相微萃取的配体捕捞方法

效应导向分析 (EDA) 越来越多地用于筛选环境中的有毒污染物，但传统的 EDA 程序通常耗时且费力。这对在需要快速答案的情况下使用 EDA 进行有毒物质识别提出了挑战。在此，提出了固相微萃取配体捕捞 (SPME-LF) 策略作为快速 EDA 方法，用于鉴定水中的乙酰胆碱酯酶 (AChE) 活性化合物。首先通过膜分离方法验证了配体捕捞技术从环境混合物中筛选乙酰胆碱酯酶活性化学物质的可行性。然后，通过硼酸基团与AChE共键合自组装制备SPME纤维，并将其应用于SPME-LF。由于 AChE 涂层 SPME 纤维选择性地从水中富集 AChE 活性化合物，因此比较有和没有 AChE 涂层的 SPME 纤维吸附的化合物可以快速区分混合物中的 AChE 毒物。与传统的EDA相比，SPME-LF不需要重复的样品分离和生物测定，赋予SPME-LF低成本、省力、用户友好的优点。人们相信，成本效益高且易于使用的 SPME-LF 策略可能成为一种快速 EDA 方法，用于筛选水生环境中受体特异性毒物，特别适用于时间敏感的筛选。