This paper discusses a microfluidic system designed for surface plasmon resonance (SPR) sensing, incorporating integrated microvalves. This system is built from a layered structure of polydimethylsiloxane (PDMS) and polymethylmethacrylate (PMMA). The functionality of the microvalves is verified through a conductance method involving electrodes positioned at the microfluidic channels’ inlets and outlets. These microvalves can fully close at a control pressure of 0.3 MPa, with their operation depending on the duration of the applied pressure. The study further explores the coordinated operation of multiple microvalves to regulate the sequential flow of samples and reagents in the system. In SPR detection experiments, the microfluidic system is integrated with an SPR array sensing system to control the injection of NaCl solutions via the microvalves, and the observation of phase change curves in different chip regions are observed. The findings validate the microvalves’ dependability and suitability for use in SPR array sensing.

中文翻译：

---

微型阀集成SPR阵列传感芯片的实验研究

本文讨论了一种专为表面等离子共振 (SPR) 传感而设计的微流体系统，其中包含集成微阀。该系统由聚二甲基硅氧烷（PDMS）和聚甲基丙烯酸甲酯（PMMA）的层状结构构成。微阀的功能通过电导方法进行验证，该方法涉及位于微流体通道入口和出口的电极。这些微型阀可以在 0.3 MPa 的控制压力下完全关闭，其操作取决于施加压力的持续时间。该研究进一步探索了多个微型阀的协调操作，以调节系统中样品和试剂的顺序流动。在SPR检测实验中，微流控系统与SPR阵列传感系统集成，通过微阀控制NaCl溶液的注入，并观察不同芯片区域的相变曲线。研究结果验证了微型阀在 SPR 阵列传感中的可靠性和适用性。