This study introduces a paradigm-shifting approach to optimize mitochondrial targeting. Employing a new fluorescent probe strategy, we unravel a combined influence of both Nernst potential (Ψ) and partitioning (P) contributions. Through the synthesis of new benz[e]indolinium-derived probes, our findings redefine the landscape of mitochondrial localization by optimizing the efficacy of mitochondrial probe retention in primary cortical neurons undergoing normoxia and oxygen–glucose deprivation. This methodology not only advances our understanding of subcellular dynamics, but also holds promise for transformative applications in biomedical research and therapeutic development.

中文翻译：

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探索线粒体靶向：创新的荧光探针揭示能斯特势和分配组合

这项研究引入了一种范式转换方法来优化线粒体靶向。采用新的荧光探针策略，我们揭示了能斯特势 ( Ψ ) 和分配 ( P ) 贡献的综合影响。通过合成新的苯并[ e ]吲哚衍生探针，我们的研究结果通过优化线粒体探针在常氧和氧糖剥夺的原代皮质神经元中保留的功效，重新定义了线粒体定位的前景。这种方法不仅增进了我们对亚细胞动力学的理解，而且有望在生物医学研究和治疗开发中实现变革性应用。