The rodent-borne Arenavirus in humans has led to the emergence of regional endemic situations and has deeply emerged into pandemic-causing viruses. Arenavirus have a bisegmented ambisense RNA that produces four proteins: glycoprotein, nucleocapsid, RdRp and Z protein. The peptide-based vaccine targets the glycoprotein of the virus encountered by the immune system. Screening of B-Cell and T-Cell epitopes was done based on their immunological properties like antigenicity, allergenicity, toxicity and anti-inflammatory properties were performed. Selected epitopes were then clustered and epitopes were stitched using linker sequences. The immunological and physico-chemical properties of the vaccine construct was checked and modelled structure was validated by a 2-step MD simulation. The thermostability of the vaccine was checked followed by the immune simulation to test the immunogenicity of the vaccine upon introduction into the body over the course of the next 100 days and codon optimization was performed. Finally a 443 amino acid long peptide vaccine was designed which could provide protection against several members of the mammarenavirus family in a variety of population worldwide as denoted by the epitope conservancy and population coverage analysis. This study of designing a peptide vaccine targeting the glycoprotein of mammarenavirues may help develop novel therapeutics in near future.

人类中啮齿类动物传播的沙粒病毒已导致区域性流行情况的出现，并已深入发展成为引起大流行的病毒。沙粒病毒具有双片段双义 RNA，可产生四种蛋白质：糖蛋白、核衣壳、RdRp 和 Z 蛋白。基于肽的疫苗针对免疫系统遇到的病毒糖蛋白。 B 细胞和 T 细胞表位的筛选是根据其免疫学特性（如抗原性、过敏性、毒性和抗炎特性）进行的。然后对选定的表位进行聚类，并使用接头序列缝合表位。检查了疫苗构建体的免疫学和物理化学特性，并通过两步 MD 模拟验证了模型结构。检查疫苗的热稳定性，然后进行免疫模拟，以测试疫苗在接下来的 100 天内引入体内后的免疫原性，并进行密码子优化。最后设计了一种 443 个氨基酸的长肽疫苗，如表位保守性和人群覆盖率分析所示，它可以为世界各地不同人群中的乳腺病毒家族的几种成员提供保护。这项设计针对哺乳动物病毒糖蛋白的肽疫苗的研究可能有助于在不久的将来开发新的治疗方法。