D-phenyllactic acid is an important medical intermediate and a potential green preservative. Due to its extensive use and high demand, there has been significant research interest in developing efficient synthesis methods. In this study, we aimed to efficiently produce -phenyllactic acid from -phenylalanine through a two-step cascade reaction using a coupled catalytic system consisting of two bacteria strains (/pET28a- and /pET28a-). To enhance the stability of this catalytic system, we encapsulated the two recombinant strains with amorphous ZIF-90, which was thoroughly characterized using various methods including SEM, FT-IR, TGA, XRD, XPS, and CLSM analysis. Compared to free cells, the immobilized living materials (amorphous ZIF-90@) showed good stability in harsh environments and superior recyclability. When applied to the production of -PLA, the amorphous ZIF-90@ system achieved a yield of 9.00 g/L and a conversion of 89.4 %. This successful coupling catalysis from L-phenylalanine to -PLA indicates the potential of aZIF-90@ for biosynthesis of -phenyllactic acid in future industrial applications.

中文翻译：

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利用非晶态金属有机骨架修饰大肠杆菌级联合成D-苯基乳酸

D-苯基乳酸是重要的医药中间体和潜在的绿色防腐剂。由于其广泛的用途和高需求，人们对开发有效的合成方法产生了浓厚的研究兴趣。在这项研究中，我们的目标是使用由两种细菌菌株（/pET28a-和/pET28a-）组成的耦合催化系统，通过两步级联反应，有效地从-苯丙氨酸生产-苯基乳酸。为了增强该催化系统的稳定性，我们用无定形 ZIF-90 封装了两种重组菌株，并使用 SEM、FT-IR、TGA、XRD、XPS 和 CLSM 分析等各种方法对其进行了彻底表征。与游离细胞相比，固定化活材料（非晶ZIF-90@）在恶劣环境中表现出良好的稳定性和优异的可回收性。当应用于-PLA的生产时，非晶态ZIF-90@体系的产率达到9.00 g/L，转化率达到89.4%。这种从L-苯丙氨酸到-PLA的成功偶联催化表明aZIF-90@在未来工业应用中用于生物合成-苯乳酸的潜力。