Transglutaminase 2 (TG2) performs many functions both under physiological and pathological conditions. In cancer, its expression is associated with aggressiveness, propensity to epithelial-mesenchymal transition, and metastasis. Since TG2 performs key functions both outside and inside the cell, using inhibitors with different membrane permeability we analyzed the changes in the transcriptome induced in two triple-negative cell lines (MDA-MB-436 and MDA-MB-231) with aggressive features. By characterizing pathways and gene networks, we were able to define the effects of TG2 inhibitors (AA9, membrane-permeable, and NCEG2, impermeable) in relation to the roles of the enzyme in the intra- and extracellular space within the context of breast cancer.

中文翻译：

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使用膜渗透性和非渗透性抑制剂进行转录组学研究揭示乳腺癌细胞中转谷氨酰胺酶 2 的功能

转谷氨酰胺酶 2 (TG2) 在生理和病理条件下均发挥多种功能。在癌症中，其表达与侵袭性、上皮间质转化倾向和转移相关。由于 TG2 在细胞外部和内部均发挥关键功能，因此我们使用具有不同膜渗透性的抑制剂分析了两种具有侵袭性特征的三阴性细胞系（MDA-MB-436 和 MDA-MB-231）中诱导的转录组变化。通过表征途径和基因网络，我们能够定义 TG2 抑制剂（AA9，膜可渗透的，NCEG2，不可渗透的）与乳腺癌背景下酶在细胞内和细胞外空间中的作用的关系。