Abstract

A two-section analytical chamber was developed and tested for study of EA.hy926 endothelial cells culture in real time with high resolution. It has been shown that the method of scanning ion-conductance microscopy yields relevant results, since it ensures the absence of mechanical effects on cells, and allows to scan endothelial cells on membranes that ensures that cells are surrounded by a nutrient medium. The method made it possible not only to visualize changes in the cell surface during long-term scanning, but also to detect extracellular (microfilaments) and intracellular (nucleolus) structures. The creating of a rigidity map allowed it to be estimated that the mean rigidity of endothelial cell membranes was in the range from 357 to 796 Pa. After 240 min from the start of observation, the endothelial cells began to produce apoptotic bodies, wherein the rigidity of the cells gradually increased and the rigidity of the apoptotic bodies decreased.

中文翻译：

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利用原子力和扫描离子电导显微镜研究 EA.hy926 内皮细胞

摘要

开发并测试了两部分分析室，用于高分辨率实时研究 EA.hy926 内皮细胞培养。事实证明，扫描离子电导显微镜方法可以产生相关结果，因为它确保了细胞不存在机械效应，并且可以扫描膜上的内皮细胞，从而确保细胞被营养培养基包围。该方法不仅可以在长期扫描过程中可视化细胞表面的变化，还可以检测细胞外（微丝）和细胞内（核仁）结构。刚性图的创建使得可以估计内皮细胞膜的平均刚性在357至796Pa的范围内。从观察开始240分钟后，内皮细胞开始产生凋亡小体，其中刚性细胞的数量逐渐增多，凋亡小体的刚性下降。