Abstract

The amniotic variant of glycodelin (GdA) has pronounced immunomodulatory properties, being involved in the formation of immune tolerance during pregnancy. The effect of GdA on the amount of T-regulatory lymphocytes (Treg) and the level of acute phase proteins (α-2-macroglobulin (α-2M), orosomucoid, C-reactive protein (CRP)) were studied during the administration of allogeneic red bone marrow (BM) cells to Wistar rats in a dynamic in vivo experiment. It was established that the administration of GdA against the background of the administration of allogeneic BM caused an increase in the portion of peripheral Tregs among CD4⁺ lymphocytes at the end of the experiment (on the 21st day) as compared with the group, which received BM. It was demonstrated that GdA decreased the level of CRP and α-2M, but increased the orosomucoid concentration in the serum of experimental animals at the beginning of the experiment (third day); however, normalization of the content of acute phase proteins to the level of intact animals was observed by the end of the experiment (21st day) in all groups of experimental animals. Thus, glycodelin is able to realize an immunosuppressive effect relative to the allogeneic cells through an increase in the level of Treg and orosomucoid, as well as a decrease in the concentration of CRP and α-2M.

中文翻译：

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甘氨肽对同种异体骨髓细胞给药期间Wistar大鼠调节性T淋巴细胞和急性期蛋白水平影响的研究

摘要

羊膜糖肽 (GdA) 具有显着的免疫调节特性，参与妊娠期间免疫耐受的形成。研究了给药过程中 GdA 对调节性 T 淋巴细胞 (Treg) 数量和急性期蛋白（α-2-巨球蛋白 (α-2M)、口腔黏液、C 反应蛋白 (CRP)）水平的影响。在动态体内实验中将同种异体红骨髓 (BM) 细胞移植到 Wistar 大鼠体内。确定在给予同种异体BM的背景下给予GdA导致实验结束时（第21天）与接受GdA治疗的组相比， CD4 ⁺淋巴细胞中外周Treg的比例增加。 BM。证明GdA在实验开始时（第三天）降低了实验动物血清中CRP和α-2M的水平，但增加了orosumucoid的浓度；然而，在实验结束时（第21天），在所有实验动物组中都观察到急性期蛋白含量正常化至完整动物的水平。因此，Glydelin能够通过增加Treg和orosumucoid的水平以及降低CRP和α-2M的浓度来实现相对于同种异体细胞的免疫抑制作用。