Glomerella leaf spot (GLS), a fungal disease caused by Colletotrichum fructicola, severely affects apple (Malus domestica) quality and yield. In this study, we found that the transcription factor MdWRKY71 was significantly induced by C. fructicola infection in the GLS‐susceptible apple cultivar Royal Gala. The overexpression of MdWRKY71 in apple leaves resulted in increased susceptibility to C. fructicola, whereas RNA interference of MdWRKY71 in leaves showed the opposite phenotypes. These findings suggest that MdWRKY71 functions as a susceptibility factor for the apple—C. fructicola interaction. Furthermore, MdWRKY71 directly bound to the promoter of the salicylic acid (SA) degradation gene Downy Mildew Resistant 6 (DMR6)‐Like Oxygenase 1 (DLO1) and promoted its expression, resulting in a reduced SA level. The sensitivity of 35S:MdWRKY71 leaves to C. fructicola can be effectively alleviated by knocking down MdDLO1 expression, confirming the critical role of MdWRKY71‐mediated SA degradation via regulating MdDLO1 expression in GLS susceptibility. In summary, we identified a GLS susceptibility factor, MdWRKY71, that targets the apple SA degradation pathway to promote fungal infection.

中文翻译：

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MdWRKY71通过控制水杨酸降解促进苹果对肾小球叶斑病的敏感性

球小球叶斑病 (GLS)，一种由球菌引起的真菌病害果炭疽菌，严重影响苹果（海棠）质量和产量。在这项研究中，我们发现转录因子MdWRKY71被显着诱导果果红藻GLS 易感苹果品种 Royal Gala 的感染。的过度表达麦德WRKY71在苹果叶中导致对以下疾病的易感性增加果果红藻，而 RNA 干扰麦德WRKY71叶子中表现出相反的表型。这些发现表明 MdWRKY71 是苹果的一个易感因子——果果红藻相互作用。此外，MdWRKY71直接结合水杨酸（SA）降解基因的启动子抗霜霉病6（DMR6）‐如加氧酶 1（DLO1）并促进其表达，导致SA水平降低。 35S灵敏度：麦德WRKY71离开到果果红藻通过打倒可以有效缓解MdDLO1表达，证实了 MdWRKY71 通过调节介导的 SA 降解的关键作用MdDLO1GLS 敏感性的表达。总之，我们确定了一个 GLS 易感因子 MdWRKY71，它针对苹果 SA 降解途径来促进真菌感染。