Phytophthora root and stem rot of soybean (Glycine max), caused by the oomycete Phytophthora sojae, is an extremely destructive disease worldwide. In this study, we identified GmEIL1, which encodes an ethylene‐insensitive3 (EIN3) transcription factor. GmEIL1 was significantly induced following P. sojae infection of soybean plants. Compared to wild‐type soybean plants, transgenic soybean plants overexpressing GmEIL1 showed enhanced resistance to P. sojae and GmEIL1‐silenced RNA‐interference lines showed more severe symptoms when infected with P. sojae. We screened for target genes of GmEIL1 and confirmed that GmEIL1 bound directly to the GmERF113 promoter and regulated GmERF113 expression. Moreover, GmEIL1 positively regulated the expression of the pathogenesis‐related gene GmPR1. The GmEIL1‐regulated defence response to P. sojae involved both ethylene biosynthesis and the ethylene signalling pathway. These findings suggest that the GmEIL1‐GmERF113 module plays an important role in P. sojae resistance via the ethylene signalling pathway.

中文翻译：

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EIN3转录因子GmEIL1提高大豆对大豆疫霉的抗性

大豆疫霉根腐病（大豆），由卵菌引起大豆疫霉，是一种世界范围内极具破坏性的疾病。在这项研究中，我们确定了基因EIL1，编码乙烯不敏感 3 (EIN3) 转录因子。基因EIL1显着诱导以下酱油大豆植物的感染。与野生型大豆植物相比，转基因大豆植物过度表达基因EIL1显示出增强的抵抗力酱油和基因EIL1‐沉默的RNA干扰系在感染时表现出更严重的症状酱油。我们筛选了GmEIL1的靶基因，并证实GmEIL1直接与基因ERF113发起者和监管者基因ERF113表达。此外，GmEIL1正向调节发病相关基因的表达基因PR1。 GmEIL1 调节的防御反应酱油涉及乙烯生物合成和乙烯信号通路。这些发现表明 GmEIL1‐基因ERF113模块发挥着重要作用酱油通过乙烯信号通路产生耐药性。