A versatile method is invented to self‐assemble gold nanoparticles (GNPs) into nanoclusters (NCs) of various morphologies. By storing the particles in toluene, a highly non‐polar solvent, under conditions that ensure particle stability, the success rate of subsequent assembly can be enhanced. Additionally, conducting particle self‐assembly at a stirring speed of 200 rpm allows the NCs to maintain a spherical shape. The relative standard deviation (RSD) of Raman spectral peaks of multiple NCs used as surface‐enhanced Raman spectroscopy (SERS) substrates is calculated to be less than 10%, effectively addressing the issue of low repeatability when using NCs as SERS substrates. Furthermore, even at an analyte concentration reduced to 10−9 m, a SERS characteristic peak intensity of approximately 2 × 103 is measurable, demonstrating the high sensitivity of the assembled structures. Finally, by detecting SERS signals from NCs of varying sizes, the intensities of characteristic peaks tend to converge, eliminating the influence of morphology and size on SERS detection.

中文翻译：

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表面修饰纳米粒子自组装成 SERS 活性纳米团簇的通用方法

发明了一种通用方法，可将金纳米颗粒（GNP）自组装成各种形态的纳米团簇（NC）。通过将颗粒储存在高度非极性溶剂甲苯中，在确保颗粒稳定性的条件下，可以提高后续组装的成功率。此外，在 200 rpm 的搅拌速度下进行颗粒自组装可以使 NC 保持球形。经计算，多个NC作为表面增强拉曼光谱（SERS）基底的拉曼光谱峰的相对标准偏差（RSD）小于10%，有效解决了NC作为SERS基底时重复性低的问题。此外，即使分析物浓度降低至 10−9 米，SERS 特征峰强度约为 2 × 103是可测量的，证明了组装结构的高灵敏度。最后，通过检测不同尺寸NC的SERS信号，特征峰的强度趋于收敛，消除了形态和尺寸对SERS检测的影响。