Nanosecond pulsed electric fields (nsPEFs) have recently shown promise as a cancer therapy. Although nsPEF‐induced apoptotic responses have been observed, the stress on individual cell components has not been quantified. Therefore, the authors built a cell equivalent circuit, including mitochondria, to quantitatively calculate the voltage, electric field, and electrical energy applied to the cell components in the frequency domain. Additionally, pulse width and electrical energy changed in mitochondrial membrane potential over time. The results indicated that the change of pulsed voltage switched the cell stimulation pathway, resulting in different mitochondrial membrane potentials over time. The frequency response analysis confirmed that the potential on mitochondrial membranes increased under shorter pulse width conditions. This suggested that the frequency analysis provided in this paper is useful for relating the site of cellular stimulation to induce physiological effects. © 2024 Institute of Electrical Engineer of Japan and Wiley Periodicals LLC.

中文翻译：

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低压纳秒脉冲电场的脉宽和电能对癌细胞线粒体的影响

纳秒脉冲电场 (nsPEF) 最近显示出作为癌症治疗的前景。尽管已观察到 nsPEF 诱导的细胞凋亡反应，但对单个细胞成分的压力尚未量化。因此，作者构建了一个包括线粒体在内的细胞等效电路，以定量计算频域中施加到细胞组件上的电压、电场和电能。此外，线粒体膜电位的脉冲宽度和电能随着时间的推移而变化。结果表明，脉冲电压的变化改变了细胞刺激途径，导致线粒体膜电位随时间变化。频率响应分析证实，在较短的脉冲宽度条件下，线粒体膜上的电位增加。这表明本文提供的频率分析对于关联细胞刺激部位以诱导生理效应是有用的。 © 2024 日本电气工程师协会和 Wiley periodicals LLC。