Introduction Feline panleukopenia is a contagious viral disease caused by the feline panleukopenia virus (FPV). A closely related pathogen is canine parvovirus (CPV), and amino acid substitutions in this virus allow it to acquire a feline host range. In feline hosts, the disease induced by CPV manifests with similar symptoms to those caused by FPV or milder ones, leading to its underdiagnosis. The aim of this study was to determine the presence of CPV type 2 (CPV-2) in cats with clinical symptoms of panleukopenia and to assess the use of commercial CPV antigen tests for the clinical diagnosis of FPV. Material and Methods Samples from 59 cats from central Slovakia were included in the study. Rectal swabs were collected and clinically tested for parvovirus infection using a commercial antigen test. Antigen-positive samples were confirmed by PCR targeting the viral VP2 gene. The sequences of the PCR products were established with the Sanger method. Results Of 59 samples, 23 were revealed to be positive for parvovirus infection by both antigen and PCR test (38.9%). Analysis with the National Center for Biotechnology Information BLASTn application showed 99.78–100% pairwise identity with FPV. The mortality rate of parvovirus-infected cats included in this study was 8.69% (2/23). Conclusion Although feline disease with CPV-2 was not confirmed, the CPV antigen test was able to detect FPV infection.

中文翻译：

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斯洛伐克有猫泛白细胞减少症临床症状的猫中犬细小病毒发生情况的调查——初步研究

简介 猫泛白细胞减少症是由猫泛白细胞减少症病毒（FPV）引起的传染性病毒性疾病。一种密切相关的病原体是犬细小病毒（CPV），这种病毒中的氨基酸取代使其能够获得猫科动物的宿主范围。在猫宿主中，由 CPV 引起的疾病表现出与 FPV 引起的症状相似或更轻微的症状，导致其诊断不足。本研究的目的是确定具有全白细胞减少症临床症状的猫是否存在 2 型 CPV (CPV-2)，并评估商业 CPV 抗原测试在 FPV 临床诊断中的应用。材料和方法 研究中包括来自斯洛伐克中部 59 只猫的样本。收集直肠拭子并使用商业抗原测试对细小病毒感染进行临床测试。通过针对病毒的 PCR 确认抗原阳性样本副总裁2基因。 PCR产物的序列采用Sanger法建立。结果 59份样本中，抗原和PCR检测均呈细小病毒感染阳性23份（38.9%）。国家生物技术信息中心 BLASTn 应用程序的分析显示与 FPV 的配对一致性为 99.78–100%。本研究纳入的细小病毒感染猫的死亡率为8.69%（2/23）。结论 虽然未证实猫患有 CPV-2 疾病，但 CPV 抗原检测能够检测 FPV 感染。