There is an urgent need to improve conventional cancer‐treatments by preventing detrimental side effects, cancer recurrence and metastases. Recent studies have shown that presence of senescent cells in tissues treated with chemo‐ or radiotherapy can be used to predict the effectiveness of cancer treatment. However, although the accumulation of senescent cells is one of the hallmarks of cancer, surprisingly little progress has been made in development of strategies for their detection in vivo. To address a lack of detection tools, we developed a biocompatible, injectable organic nanoprobe (NanoJagg), which is selectively taken up by senescent cells and accumulates in the lysosomes. The NanoJagg probe is obtained by self‐assembly of indocyanine green (ICG) dimers using a scalable manufacturing process and characterized by a unique spectral signature suitable for both photoacoustic tomography (PAT) and fluorescence imaging. In vitro, ex vivo and in vivo studies all indicate that NanoJaggs are a clinically translatable probe for detection of senescence and their PAT signal makes them suitable for longitudinal monitoring of the senescence burden in solid tumors after chemotherapy or radiotherapy.

中文翻译：

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用于细胞衰老体内检测的吲哚菁绿纳米探针

迫切需要通过预防有害副作用、癌症复发和转移来改进传统癌症治疗。最近的研究表明，接受化疗或放疗的组织中是否存在衰老细胞，可以用来预测癌症治疗的有效性。然而，尽管衰老细胞的积累是癌症的标志之一，但令人惊讶的是，在开发体内检测策略方面却几乎没有取得进展。为了解决检测工具的缺乏，我们开发了一种生物相容性、可注射的有机纳米探针（NanoJagg），它被衰老细胞选择性地吸收并在溶酶体中积累。 NanoJagg 探针是通过使用可扩展的制造工艺自组装吲哚菁绿 (ICG) 二聚体而获得的，其特点是具有适合光声断层扫描 (PAT) 和荧光成像的独特光谱特征。体外、离体和体内研究均表明 NanoJaggs 是一种用于检测衰老的临床可翻译探针，其 PAT 信号使其适合纵向监测化疗或放疗后实体瘤的衰老负担。