BackgroundOne significant component of new, rapid economic development is the creation of biodegradable polymers. One of these biopolymers is polyhydroxyalkanoate (PHA). This biodegradable, thermoplastic and water insoluble storage polymer can be generated from sustainable carbon sources. PHA may be effectively used for treating wastewaters by immobilizing enzymes. For eliminating micro‐pollutants along with many phenolic compounds, laccase is considered as a potential enzyme. Hence, it has been known to be highly effective for water purification procedures. However, adequate immobilization is required for laccase to perform efficient catalysis. The immobilization procedure increases laccase stability with respect to reusability, temperature, pH and storage, making it superior to free laccase.ResultIn the present study, laccase was partially purified from Beauveria pseudobassiana PHF4 by gel filtration chromatography and further immobilized on PHA microbeads. The crude extract displayed specific activity of 19.08 U/mg and PHA microbeads demonstrated immobilization efficiency of 77.44%. Characterization of PHA microbeads by scanning electron microscopy showed increase in their size from 3–5 μm to 5–6 μm after the immobilization. Furthermore, catechol biodegradation by immobilized laccase was analyzed using UV–Vis spectrophotometry (84.25% in 10 h), also confirmed by high performance liquid chromatography (83.65% in 24 h).ConclusionHence by immobilizing laccase on PHA microbeads, the pollution and environmental damage that catechol and similar chemicals cause, can be addressed while also developing ecofriendly solutions.This article is protected by copyright. All rights reserved.

中文翻译：

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部分纯化漆酶在聚羟基脂肪酸酯（PHA）微珠上的固定化及其在儿茶酚生物降解中的应用

背景新的、快速的经济发展的一个重要组成部分是生物可降解聚合物的创造。这些生物聚合物之一是聚羟基链烷酸酯（PHA）。这种可生物降解、热塑性和水不溶性存储聚合物可以由可持续的碳源产生。 PHA可通过固定化酶有效地用于处理废水。为了消除微污染物和许多酚类化合物，漆酶被认为是一种潜在的酶。因此，众所周知，它对于水净化过程非常有效。然而，漆酶需要充分的固定才能进行有效的催化。固定化程序提高了漆酶在可重复使用性、温度、pH 和储存方面的稳定性，使其优于游离漆酶。结果在本研究中，漆酶部分纯化自白僵菌PHF4通过凝胶过滤层析进一步固定在PHA微珠上。粗提物的比活性为19.08 U/mg，PHA微珠的固定化效率为77.44%。通过扫描电子显微镜对 PHA 微珠进行表征表明，固定后其尺寸从 3-5 μm 增加到 5-6 μm。此外，使用紫外-可见分光光度法分析了固定化漆酶对儿茶酚的生物降解作用（10小时内84.25％），并通过高效液相色谱法进行了证实（24小时内83.65％）。结论因此，通过将漆酶固定在PHA微珠上，污染和环境破坏邻苯二酚和类似化学物质引起的问题可以在开发环保解决方案的同时得到解决。本文受版权保护。版权所有。