Post-transcriptional regulation has emerged as a key mechanism to regulate stem cell renewal and differentiation, which is essential for understanding tissue regeneration and homeostasis. Poly(A)-binding proteins are a family of RNA-binding proteins that play a vital role in post-transcriptional regulation by controlling mRNA stability and protein synthesis. The involvement of poly(A) binding proteins in a wide range of cellular functions is increasingly being investigated. In this study, we used the regenerative model organism planarian Schmidtea mediterranea, to demonstrate the critical role of poly(A)-binding protein 2 (PABP2) in regulating neoblast maintenance and differentiation. A deficit in PABP2 blocks the transition of neoblasts towards immediate early progenitors, leading to an enhanced pool of non-committed neoblasts and a decreased progenitor population. This is reflected in variations in the transcriptome profile, providing evidence of downregulation in multiple lineages. Thus, insufficiency of PABP2 resulted in defective formation and organization of tissue leading to abnormal regeneration. Our study reveals the essential role of PABP2 in regulating genes that mediate stem cell commitment to early progenitors during tissue regeneration.

中文翻译：

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Poly (A) 结合蛋白 2 对于涡虫 Schmidtea mediterranea 再生过程中的干祖细胞分化至关重要。

转录后调控已成为调节干细胞更新和分化的关键机制，这对于理解组织再生和稳态至关重要。 Poly(A) 结合蛋白是 RNA 结合蛋白家族，通过控制 mRNA 稳定性和蛋白质合成，在转录后调控中发挥重要作用。人们越来越多地研究多聚腺苷酸结合蛋白在多种细胞功能中的作用。在这项研究中，我们使用再生模型生物涡虫 Schmidtea mediterranea 来证明多聚腺苷酸结合蛋白 2 (PABP2) 在调节新生细胞维持和分化中的关键作用。 PABP2的缺陷阻碍了新生细胞向直接早期祖细胞的转变，导致未定型的新生细胞数量增加和祖细胞数量减少。这反映在转录组谱的变化中，提供了多个谱系下调的证据。因此，PABP2不足会导致组织形成和组织缺陷，从而导致异常再生。我们的研究揭示了 PABP2 在调节组织再生过程中介导干细胞向早期祖细胞定向的基因中的重要作用。