(Muscle Segment Homeobox 1) has pleiotropic effects in various tissues, including cardiomyocytes, while the effect of on cardiomyocyte cellular function was not well known. In this study, we used AC16 cell culture, real-time fluorescence quantitative PCR (qPCR), protein blotting (Western blot), flow cytometry apoptosis assay and lactate dehydrogenase (LDH) ELISA (Enzyme-Linked Immunosorbnent Assay) to investigate the effect of the gene on cardiomyocyte function. The results showed that plays a protective role against hypoxia of cardiomyocytes. However, further studies are required to fully understand the role of in the regulation of LDH expression in different cell types and under different conditions.

中文翻译：

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MSX1对心肌细胞功能的影响

(Muscle Segment Homeobox 1) 在包括心肌细胞在内的各种组织中具有多效性，但对心肌细胞功能的影响尚不清楚。在本研究中，我们采用AC16细胞培养、实时荧光定量PCR（qPCR）、蛋白印迹（Western blot）、流式细胞术凋亡测定和乳酸脱氢酶（LDH）ELISA（酶联免疫吸附测定）来研究影响心肌细胞功能的基因。结果表明对心肌细胞缺氧具有保护作用。然而，需要进一步的研究来充分了解不同细胞类型和不同条件下LDH表达的调节作用。