, a plant of great medicinal value found in cold high-altitude regions, has been excessively exploited due to the difficulty in cultivation. Understanding 's adaptation mechanisms to cold environment can provide a theoretical basis for artificial breeding. Glutathione peroxidases (GPXs), critical enzymes found in plants, play essential roles in antioxidant defense through the ascorbate–glutathione cycle. However, it is unknown whether contributes to 's cold tolerance. In this study, we investigated the role of in 's cold tolerance mechanisms. By overexpressing () in yeast and , we observed down-regulation of () and increased cold tolerance in both organisms. Furthermore, the levels of antioxidants and enzyme activities in the ascorbate–glutathione cycle were elevated, and cold-responsive genes such as and were induced. Additionally, overexpressing lines showed insensitivity to exogenous abscisic acid (ABA), suggesting a negative regulation of the ABA pathway by . also promoted the expression of several thioredoxin genes in and interacted with two endogenous genes of , and , located in mitochondria and chloroplasts. These findings suggest a significantly different model in compared to , highlighting a complex network involving the function of . Moreover, overexpressing in hairy roots positively influenced the salidroside synthesis pathway, enhancing its pharmaceutical value for doxorubicin-induced cardiotoxicity. These results suggested that might be a key component for to adapt to cold stress and overexpressing could enhance the pharmaceutical value of the hairy roots.

中文翻译：

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红景天谷胱甘肽过氧化物酶5的过度表达增加了毛状根的耐寒性并增强了药用价值

这种产于寒冷高海拔地区的具有极高药用价值的植物，由于栽培困难而被过度开发。了解其对寒冷环境的适应机制可为人工育种提供理论依据。谷胱甘肽过氧化物酶 (GPX) 是植物中发现的关键酶，通过抗坏血酸-谷胱甘肽循环在抗氧化防御中发挥重要作用。但是否有助于其耐寒性尚不清楚。在这项研究中，我们研究了 的耐冷机制的作用。通过在酵母和 中过度表达 ()，我们观察到两种生物体中 () 的下调和耐冷性的增加。此外，抗坏血酸-谷胱甘肽循环中的抗氧化剂和酶活性水平升高，并且诱导了冷反应基因，例如 和 。此外，过表达株系对外源脱落酸 (ABA) 不敏感，表明 ABA 途径存在负调控。还促进位于线粒体和叶绿体中的两个内源基因 和 的几个硫氧还蛋白基因的表达并与其相互作用。这些发现表明，与 相比，模型存在显着不同，强调了涉及 的功能的复杂网络。此外，毛状根中的过度表达对红景天苷合成途径产生积极影响，增强其对阿霉素诱导的心脏毒性的药用价值。这些结果表明，它可能是适应冷应激的关键成分，过度表达可以提高毛状根的药用价值。