DEAD-box helicases are important players in mitochondrial gene expression, which is necessary for mitochondrial respiration. In this study, we characterized Mss116 (spMss116), a member of the family of DEAD-box RNA helicases. Deletion of in a mitochondrial intron-containing background significantly reduced the levels of mitochondrial DNA (mtDNA)-encoded and mRNAs and impaired mitochondrial translation, leading to a severe respiratory defect and a loss of cell viability during stationary phase. Deletion of mitochondrial introns restored the levels of and mRNAs to wide-type (WT) levels but could not restore mitochondrial translation and respiration in Δ cells. Furthermore, deletion of in both mitochondrial intron-containing and intronless backgrounds impaired mitoribosome assembly and destabilization of mitoribosomal proteins. Our findings suggest that defective mitochondrial translation caused by deletion of is most likely due to impaired mitoribosome assembly.

中文翻译：

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粟酒裂殖酵母 DEAD-box 蛋白 Mss116 是线粒体核糖体组装和线粒体翻译所必需的

DEAD-box解旋酶是线粒体基因表达的重要参与者，而线粒体基因表达是线粒体呼吸所必需的。在这项研究中，我们鉴定了 Mss116 (spMss116)，它是 DEAD-box RNA 解旋酶家族的成员。含有线粒体内含子的背景中的删除显着降低了线粒体 DNA (mtDNA) 编码和 mRNA 的水平，并损害了线粒体翻译，导致严重的呼吸缺陷和稳定期细胞活力的丧失。线粒体内含子的删除将 和 mRNA 的水平恢复到宽型 (WT) 水平，但不能恢复 Δ 细胞中的线粒体翻译和呼吸。此外，线粒体含内含子和无内含子背景中的缺失都会损害线粒体核糖体的组装和线粒体核糖体蛋白的不稳定。我们的研究结果表明，由 缺失引起的线粒体翻译缺陷很可能是由于线粒体糖体组装受损所致。