IntroductionSkin cancer poses a significant health risk globally, necessitating effective and safe therapeutic interventions. Epigallocatechin‐3‐gallate (EGCG) from green tea and rosmarinic acid (RA) from herbs like rosemary offer promising anticancer properties. Combining these compounds may enhance their effectiveness, prompting the need for a reliable analytical method to quantify them.ObjectiveHerein, we present the development and validation of a high‐performance thin‐layer chromatography (HPTLC) method for concurrent quantification of EGCG and RA in lipid‐based nanoparticles and biological samples.MethodologyThe method underwent optimisation through design of experiments (DoE), resulting in the establishment of robust chromatographic conditions. The separation process utilised aluminium HPTLC plates coated with silica gel 60 F254 as the stationary phase, with the mobile phase comprising ethyl acetate, toluene, formic acid, and methanol in a ratio of 4:4:1:1 v/v.ResultsThe retention factor (Rf) values obtained were 0.38 for EGCG and 0.61 for RA. The method demonstrated linearity over a range of 100–500 ng/band for both compounds with excellent correlation coefficients. Limits of detection and quantification were determined, indicating high sensitivity. Precision evaluations revealed relative standard deviation below 2%, ensuring method reproducibility. Recovery assays in lipid‐based nanoparticles, plasma, and urine samples demonstrated excellent recoveries (96.2%–102.1%). Forced degradation studies revealed minimal degradation under various stress conditions, with photolytic degradation showing the least impact.ConclusionThe developed HPTLC method offers a rapid, sensitive, and reliable approach for quantifying EGCG and RA, laying the groundwork for their further investigation as anticancer agents alone and in combination therapies.

中文翻译：

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用于稳定性评估和同步定量脂质纳米颗粒和生物基质中表没食子儿茶素-3-没食子酸酯和迷迭香酸的稳健高效薄层色谱 (HPTLC) 方法

简介皮肤癌在全球范围内构成重大健康风险，需要有效且安全的治疗干预措施。绿茶中的表没食子儿茶素-3-没食子酸酯 (EGCG) 和迷迭香等草药中的迷迭香酸 (RA) 具有良好的抗癌特性。将这些化合物组合起来可能会增强它们的有效性，从而促使需要一种可靠的分析方法来量化它们。 目的在此，我们开发并验证了一种高性能薄层色谱 (HPTLC) 方法，用于同时定量脂质中的 EGCG 和 RA方法 该方法通过实验设计 (DoE) 进行了优化，从而建立了稳健的色谱条件。分离过程采用涂有硅胶 60 F 的铝制 HPTLC 板第254章以乙酸乙酯、甲苯、甲酸、甲醇为固定相，流动相为体积比为 4:4:1:1 的乙酸乙酯、甲苯、甲酸和甲醇。结果保留因子 (右F) EGCG 的值为 0.38，RA 的值为 0.61。该方法证明两种化合物在 100-500 ng/条带范围内呈线性，具有出色的相关系数。确定了检测和定量限，表明灵敏度高。精密度评估显示相对标准偏差低于 2%，确保了方法的重现性。基于脂质的纳米颗粒、血浆和尿液样本的回收率测定显示出优异的回收率（96.2%–102.1%）。强制降解研究表明，在各种应激条件下，降解程度最低，其中光解降解的影响最小。 结论 开发的 HPTLC 方法为量化 EGCG 和 RA 提供了一种快速、灵敏且可靠的方法，为进一步研究它们作为单独的抗癌药物和抗癌药物奠定了基础。在联合疗法中。