BackgroundNasopharyngeal carcinoma (NPC) is a multifactorial malignancy associated with both genetic and environmental factors. Polymorphic deletions of the phase I and phase II genes involved in the detoxification of potential carcinogens may be a risk factor for nasopharyngeal carcinoma. In this study, we investigated the relationship between CYP2E1 (rs3813867), CYP2A6, GSTM1(rs1183423000) and GSTT1(rs1601993659) gene variations and NPC risk in North African countries with the highest incidence of NPC (Morocco, Algeria and Tunisia). and the evaluation of the potential use of these variants as potential biomarkers for NPC management.MethodsA total of 600 NPC cases and 545 controls frequency‐matched on ethnicity, sex, age and childhood household type, were recruited from three North African countries (Morocco, Algeria and Tunisia) and analysed. Genotyping of CYP2A6 and CYP2E1(rs3813867) was performed by polymerase chain reaction restriction (PCR)‐fragment length polymorphism (RFLP) analysis and the GSTM1 (rs1183423000) and GSTT1(rs1601993659) genetic variations were evaluated using the PCR technique.ResultsThe genotype distributions of CYP2E1(rs3813867), CYP2A6, GSTM1(rs1183423000) and GSTT1(rs1601993659) genotypes did not differ significantly among NPC cases and controls (p > 0.05). Furthermore, our data did not reveal any association with smoking and the studied variants, even when the samples were stratified by the duration period of smoking.ConclusionIn this large studied North African population, our findings suggest that the functional CYP2E1, CYP2A6, GSTM1 and GSTT1 variations did not influence NPC susceptibility.

中文翻译：

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北非人群CYP2A6、CYP2E1、GSTM1、GSTT1基因的遗传变异与鼻咽癌风险

研究背景鼻咽癌（NPC）是一种与遗传和环境因素相关的多因素恶性肿瘤。参与潜在致癌物解毒的 I 相和 II 相基因的多态性缺失可能是鼻咽癌的危险因素。在这项研究中，我们调查了两者之间的关系CYP2E1（rs3813867），CYP2A6、GSTM1（rs1183423000）和GSTT1(rs1601993659) 鼻咽癌发病率最高的北非国家（摩洛哥、阿尔及利亚和突尼斯）的基因变异和鼻咽癌风险。以及评估这些变异作为鼻咽癌管理的潜在生物标志物的潜在用途。方法从三个北非国家（摩洛哥、阿尔及利亚和突尼斯）并进行了分析。基因分型CYP2A6和CYP2E1（rs3813867）通过聚合酶链反应限制性（PCR）片段长度多态性（RFLP）分析进行GSTM1（rs1183423000）和GSTT1(rs1601993659)遗传变异采用PCR技术进行评估。结果基因型分布CYP2E1(rs3813867), CYP2A6, GSTM1（rs1183423000）和GSTT1(rs1601993659)鼻咽癌病例和对照之间的基因型没有显着差异(p> 0.05）。此外，我们的数据没有揭示与吸烟和所研究的变异有任何关联，即使样本按吸烟持续时间进行分层也是如此。结论在这个大规模研究的北非人群中，我们的研究结果表明，功能性CYP2E1,CYP2A6、GSTM1和GSTT1变异不影响 NPC 易感性。