We integrate recombinase polymerase amplification (RPA) with CRISPR/Cas9-initiated nicking rolling circle amplification (CRISPR/Cas9-nRCA) for detecting Staphylococcus aureus. This approach utilizes a unique dimeric G-triplex structure, demonstrating firstly enhanced ThT fluorescence for target detection. The proof-of-concept study introduces a new avenue for integrating isothermal amplifications with CRISPR/Cas9 in the fields of pathogen detection and disease diagnosis.

中文翻译：

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在金黄色葡萄球菌检测中整合重组酶聚合酶扩增与 CRISPR/Cas9 引发的切口滚环扩增

我们将重组酶聚合酶扩增 (RPA) 与 CRISPR/Cas9 启动的切口滚环扩增 (CRISPR/Cas9-nRCA) 相结合，用于检测金黄色葡萄球菌。该方法利用独特的二聚体 G-三链体结构，首次证明了用于目标检测的增强 ThT 荧光。这项概念验证研究引入了在病原体检测和疾病诊断领域将等温扩增与 CRISPR/Cas9 相结合的新途径。