The human body hosts countless microorganisms that play diverse roles in health and disease. However, the lack of tools capable of investigating these host–microbiota interactions at large scale has left many of them undiscovered. Writing in Nature, Sonnert et al. developed and validated a tool, named BASEHIT, to map the broad interplay between bacteria and human proteins. BASEHIT identified an extensive network with thousands of newly discovered host–microbe interactions, providing insights into the microbial impact on human physiology.

As microorganisms live in the extracellular space of the human host, BASEHIT was specifically developed to examine the binding of microbial cells to extracellular and secreted proteins. The authors created a library of bacteria incubated with barcoded yeast clones, each displaying an individual human exoprotein. By isolating, sequencing and identifying bacteria–yeast complexes, BASEHIT assembled an extensive atlas of interactions spanning several tissues.

人体拥有无数微生物，它们在健康和疾病中发挥着不同的作用。然而，由于缺乏能够大规模研究这些宿主与微生物群相互作用的工具，导致其中许多问题未被发现。Sonnert 等人在《自然》杂志上撰文。开发并验证了一种名为 BASEHIT 的工具，用于绘制细菌和人类蛋白质之间广泛的相互作用。 BASEHIT 确定了一个包含数千个新发现的宿主-微生物相互作用的广泛网络，提供了有关微生物对人类生理学影响的见解。

由于微生物生活在人类宿主的细胞外空间，BASEHIT 专门用于检测微生物细胞与细胞外和分泌蛋白的结合。作者创建了一个与带有条形码的酵母克隆一起孵育的细菌文库，每个细菌都展示一个单独的人类外蛋白。通过分离、测序和鉴定细菌-酵母复合物，BASEHIT 构建了涵盖多个组织的广泛相互作用图谱。