Polycaprolactone (PCL) is an inexpensive polymer used in numerous biomedical applications due to its biocompatibility and gradual biodegradation. While PCL scaffolds with diverse topographical features are well characterized, it is challenging to functionalize PCL constructs with bioactive peptides. In this study, a thiol-norbornene click chemistry was used to immobilize thiolated peptides onto PCL surfaces. PCL was mixed with varying amounts of poly(ethylene glycol) (PEG) modified with norbornene (Nor), and the presence of Nor in PCL-Nor blends was confirmed with H NMR spectroscopy. These blends reduced PCL film surface roughness and had no effect on nanofiber diameter. Mesenchymal stem cells (MSCs) cultured on PCL-Nor films and nanofibers functionalized with thiolated RGD peptides adhered and adopted morphologies concomitant with the underlying surface topographies. This study shows that PCL-Nor blends can be used to form rigid PCL structures amenable to photopatterning with any thiolated molecule including bioactive peptides, thereby expanding the functionality of PCL-based biomaterials.

中文翻译：

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将肽共价结合到聚己内酯薄膜和纳米纤维上的简便方法

聚己内酯 (PCL) 是一种廉价的聚合物，由于其生物相容性和逐渐生物降解性，可用于多种生物医学应用。虽然具有不同形貌特征的 PCL 支架得到了很好的表征，但用生物活性肽对 PCL 结构进行功能化仍具有挑战性。在本研究中，使用硫醇-降冰片烯点击化学将硫醇化肽固定到 PCL 表面上。将 PCL 与不同量的降冰片烯 (Nor) 改性的聚乙二醇 (PEG) 混合，并通过 1H NMR 光谱证实 PCL-Nor 混合物中 Nor 的存在。这些共混物降低了 PCL 薄膜表面粗糙度，并且对纳米纤维直径没有影响。在用硫醇化 RGD 肽功能化的 PCL-Nor 薄膜和纳米纤维上培养的间充质干细胞 (MSC) 粘附并采用与下面的表面形貌相一致的形态。这项研究表明，PCL-Nor 共混物可用于形成刚性 PCL 结构，适合与包括生物活性肽在内的任何硫醇化分子进行光图案化，从而扩展基于 PCL 的生物材料的功能。