With the enhancement of environmental governance regulations, the discharge requirements for reverse osmosis wastewater have become increasingly stringent. This study proposes an innovative approach utilizing heterotrophic nitrification and aerobic denitrification (HNAD)-based biomineralization technology, combined with coconut palm silk loaded biochar, to offer a novel solution for resource-efficient and eco-friendly treatment of reverse osmosis wastewater. sp. LX16 were loaded onto modified coir silk and showed removal efficiencies of up to 97.38, 94.58, 86.24, and 100% for NH-N (65 mg L), COD (900 mg L), Ca (180 mg L), and Cd (25 mg L). Analysis of the metabolites of microorganisms reveals that coconut palm silk loaded with deciduous biochar (BCPS) not only exerts a protective effect on microorganisms, but also enhances their growth, metabolism, and electron transfer capabilities. Characterization of precipitation phenomena elucidated the mechanism of Cd removal via ion exchange, precipitation, and adsorption. Employing high-throughput and KEGG functional analyses has confirmed the biota environmental response strategies and the identification of key genes like HNAD.

中文翻译：

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基于微生物诱导钙沉淀的生物膜反应器同时去除氨氮、钙和镉：条件、机制和群落生物响应的优化

随着环境治理法规的加强，反渗透废水的排放要求也越来越严格。本研究提出了一种创新方法，利用基于异养硝化和好氧反硝化（HNAD）的生物矿化技术，结合椰丝负载生物炭，为反渗透废水的资源节约型和生态友好型处理提供新的解决方案。 sp。将 LX16 负载到改性椰壳丝上，对 NH-N (65 mg·L)、COD (900 mg·L)、Ca (180 mg·L) 和 Cd 的去除效率高达 97.38、94.58、86.24 和 100%（ 25 毫克/升）。对微生物代谢产物的分析表明，椰丝负载落叶生物炭（BCPS）不仅对微生物发挥保护作用，而且增强其生长、代谢和电子传递能力。沉淀现象的表征阐明了通过离子交换、沉淀和吸附去除镉的机制。采用高通量和 KEGG 功能分析证实了生物群环境响应策略和 HNAD 等关键基因的识别。