Abstract

Experimental results of daily counting under a microscope for 30 days of prokaryotic cells in preparations from soil samples when stained with different specific dyes and published results on daily counting of bacteria in soil using different methods are presented. The FITC dye, which stains the entire set of bacterial cells, revealed a wavelike dynamics of cell numbers with different numbers of oscillations in the form of peaks in all experiments. Using the SFDA dye, which detects only living, metabolically active cells, wavelike dynamics were also revealed, but their oscillating number was significantly less. The reliability of oscillations and differences in cell numbers when using different dyes were confirmed statistically by harmonic analysis. The wavelike dynamics of living, metabolically active cells is a consequence of the cycles of growth and death of bacterial cells and short-term trophic succession in the microbial community. External disturbing influences did not affect the manifestation of wavelike population dynamics, either in the population of living cells or in the total number of cells. The phenomenon of wavelike dynamics of nonliving bacterial cells and their numerical superiority is explained by the fact that cells, losing viability, lyse and disintegrate not immediately after dying, but with some delay in time. This leads to the accumulation and permanent superiority of the pool of dead cells when microscopically counting the total number of bacteria in the soil and explains the discrepancy in bacterial numbers between different counting methods. The presented experimental and published material will serve as a substantiation for microbiologists and biotechnologists of the need to control the dynamics of the numbers of introduced populations and communities of microorganisms into the natural environment, as well as a source of knowledge for the successful management of natural microbial communities.

中文翻译：

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土壤样品中细菌总数和代谢活性细菌的显微计数：它们的关系和数量的振荡动力学

摘要

介绍了用不同特定染料染色时在显微镜下对土壤样品制剂中的原核细胞进行 30 天每日计数的实验结果，以及已发表的使用不同方法对土壤中细菌进行每日计数的结果。 FITC 染料对整组细菌细胞进行染色，显示出细胞数量的波状动态，在所有实验中都以峰的形式出现不同数量的振荡。使用仅检测活的、代谢活跃的细胞的 SFDA 染料，也揭示了波状动力学，但它们的振荡数量明显较少。通过谐波分析统计证实了使用不同染料时振荡的可靠性和细胞数量的差异。代谢活跃的活细胞的波状动力学是细菌细胞生长和死亡循环以及微生物群落短期营养演替的结果。外部干扰影响不会影响活细胞群体或细胞总数中波状群体动态的表现。无生命细菌细胞的波状动力学现象及其数量优势可以通过以下事实来解释：失去活力的细胞在死亡后不会立即裂解和分解，而是会延迟一些时间。当用显微镜计数土壤中的细菌总数时，这导致死细胞池的积累和永久优势，并解释了不同计数方法之间细菌数量的差异。所提出的实验和发表的材料将为微生物学家和生物技术学家证明需要控制引入自然环境中的微生物种群和群落的数量动态，以及成功管理自然环境的知识来源。微生物群落。