Intracellular biosynthesis of massive quantum dots inside bacteria remains a grand challenge, with such a technology unsatisfactory due to the high toxicity of metal ions to the cell and the ultralow yield of quantum dots. Herein, a gshA-overexpressed Shewanella oneidensis MR-1 strain was constructed with the ability to effectively detoxify SeO₃²⁻ species, rendering the formation of substantial CdSe quantum dots across the entire cell entity. As such, the photoexcited electrons generated from the entire bacterium entity led to promoted electron mobilization efficiency and photoelectron-involved cellular energy metabolism, along with the direct contact between quantum dots and hydrogenase to significantly reduce electron transfer resistance. Consequently, the constructed system disclosed distinctive performance for artificial H₂ photosynthesis, with an appreciable H₂ production rate of 32.4 μM⋅h⁻¹. This work opens up new avenues for the direct biosynthesis of intracellular quantum dots across the entire microbes.

中文翻译：

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整个 S. oneidensis MR-1 中大量细胞内 CdSe QDS 的直接生物合成用于氢的光合作用

细菌内大量量子点的细胞内生物合成仍然是一个巨大的挑战，由于金属离子对细胞的高毒性和量子点的产率极低，这种技术并不令人满意。在此，构建了gshA过表达的Shewanella oneidensis MR-1菌株，其能够有效解毒SeO ₃ ²⁻物质，从而在整个细胞实体上形成大量的CdSe量子点。因此，整个细菌实体产生的光激发电子提高了电子动员效率和光电子参与的细胞能量代谢，同时量子点和氢化酶之间的直接接触显着降低了电子转移阻力。因此，所构建的系统展现了人工H ₂光合作用的独特性能，其H ₂生产率高达32.4 μM⋅h ^-1。这项工作为整个微生物细胞内量子点的直接生物合成开辟了新途径。