We comparatively evaluated the antioxidant properties of key triterpenes from Centella asiatica, including asiatic acid (AA), asiaticoside, madecassic acid, and madecassoside, in several cell types, including skin fibroblasts, macrophages, hepatocytes, and endothelial cells, under conditions promoting oxidative stress. AA conferred the highest viability on Hs68 cells exposed to ultraviolet B (UVB) irradiation. Triterpene pretreatment attenuated the UVB-induced generation of reactive oxygen species (ROS) and malondialdehyde (MDA), as well as the UVB-induced depletion of glutathione (GSH) in skin fibroblasts. AA most potently inhibited UVB-induced MMP generation, resulting in increased intracellular collagen levels. Pretreatment with triterpenes, particularly AA, significantly improved cell viability and attenuated TBHP-induced levels of ROS, alanine aminotransferase, and aspartate aminotransferase in HepG2 cells. Triterpenes attenuated ROS levels and reduced MDA and GSH expression in EA.hy926 cells. In RAW264.7 macrophages, production of nitric oxide, tumor necrosis factor-α, and interleukin-6 (indicators of LPS-induced oxidative damage) was significantly reduced by treatment with any of the triterpenes. Statistical analyses of triterpene biological activities using principal component analysis and hierarchical clustering revealed that AA exerted the greatest overall influence and showed remarkable activity in Hs68 and HepG2 cells.

中文翻译：

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积雪草四种三萜类化合物抗氧化应激能力比较

我们比较评估了积雪草中的关键三萜类化合物，包括积雪草酸 (AA)、积雪草苷、羟基积雪草酸和羟基积雪草苷，在促进氧化应激的条件下，在皮肤成纤维细胞、巨噬细胞、肝细胞和内皮细胞等多种细胞类型中的抗氧化特性。 AA 赋予暴露于紫外线 B (UVB) 照射的 Hs68 细胞最高的活力。三萜预处理减弱了 UVB 诱导的活性氧 (ROS) 和丙二醛 (MDA) 的产生，以及 UVB 诱导的皮肤成纤维细胞中谷胱甘肽 (GSH) 的消耗。 AA 最有效地抑制 UVB 诱导的 MMP 生成，导致细胞内胶原蛋白水平增加。用三萜，特别是 AA 进行预处理，显着提高了 HepG2 细胞中的细胞活力，并降低了 TBHP 诱导的 ROS、丙氨酸转氨酶和天冬氨酸转氨酶水平。三萜减弱 EA.hy926 细胞中 ROS 水平并减少 MDA 和 GSH 表达。在 RAW264.7 巨噬细胞中，使用任何三萜类化合物治疗后，一氧化氮、肿瘤坏死因子-α 和白细胞介素-6（LPS 诱导的氧化损伤的指标）的产生显着减少。使用主成分分析和层次聚类对三萜生物活性进行统计分析，结果表明AA的总体影响最大，并且在Hs68和HepG2细胞中表现出显着的活性。