Light supplementary has been widely employed in various agricultural facilities to regulate plant growth and development as well as to mediate the certain metabolic process associated with quality. Previous studies suggested that red light has a role in regulating sugar metabolism in strawberry fruit, but the underlying biological mechanism remains unknown. In this study, we investigated the effects of white and red lights on the sugar metabolism for strawberry fruit by using multi-omics and bioinformatics methods. We observed differential accumulation of D-Mannose-6-phosphate sodium salt, sorbitol, and inositol in strawberry fruits. Transcriptomic analysis revealed 3650 differentially expressed genes, and enrichment analysis of the differentially expressed genes for Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathways highlighted significant associations with phenylpropanoid biosynthetic process and galactose metabolism pathways. The results showed that 7 differentially expressed genes were involved in inositol metabolism pathway. Expression patterns of these genes were verified by qRT-PCR. Additionally, we constructed a co-expression network to identify various macromolecules including structural genes and key transcription factors involved in regulating galactinol synthase gene family members, including HD-ZIP proteins, NAC proteins, MYB proteins, and WRKY proteins, among others. Our study provides new insight into the regulation of inositol metabolism through red light supplementation in strawberry plants. Furthermore, we proposed the potential transcription factors that regulate galactinol synthase genes in inositol metabolism pathway. Our work offers theoretical support for the development of effective lighting strategies to enhance strawberry fruit quality.

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红光补充对草莓果实成熟过程中肌醇代谢的生物学作用

补光已广泛应用于各种农业设施中，以调节植物生长和发育以及调节与质量相关的某些代谢过程。先前的研究表明红光对草莓果实的糖代谢具有调节作用，但其潜在的生物学机制仍不清楚。在本研究中，我们利用多组学和生物信息学方法研究了白光和红光对草莓果实糖代谢的影响。我们观察到草莓果实中 D-甘露糖-6-磷酸钠盐、山梨醇和肌醇的积累差异。转录组分析揭示了3650个差异表达基因，对差异表达基因进行基因本体富集和京都基因和基因组百科全书途径的富集分析强调了与苯丙素生物合成过程和半乳糖代谢途径的显着关联。结果显示7个差异表达基因参与肌醇代谢途径。这些基因的表达模式通过 qRT-PCR 进行验证。此外，我们构建了一个共表达网络来识别各种大分子，包括参与调节半乳糖醇合酶基因家族成员的结构基因和关键转录因子，包括HD-ZIP蛋白、NAC蛋白、MYB蛋白和WRKY蛋白等。我们的研究为草莓植物中通过红光补充调节肌醇代谢提供了新的见解。此外，我们提出了调节肌醇代谢途径中半乳糖醇合酶基因的潜在转录因子。我们的工作为制定有效的照明策略以提高草莓果实品质提供了理论支持。