CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1), a ubiquitin E3 ligase, has been identified as a crucial repressor involved in plant development in the absence of light. However, its specific roles in lily ( ‘Viviana’) remain largely unknown. Through transcriptome analysis in lily, a gene () that positively influences lily pigmentation was identified. Evolutionary analysis revealed that is an ortholog of , subsequently designated as . The promoter region of LvCOP1 is enriched with multiple cis-acting elements responsive to light, and its expression was regulated by light, indicating that LvCOP1 plays a crucial role in response to light signals. Furthermore, the overexpression of elevates the anthocyanin content in lily petals. This enhancement is achieved by elevating the expression levels of key genes involved in biosynthesis of anthocyanin, including , and . Conversely, silencing had the opposite effect. Additionally, yeast library screening demonstrated an interaction between LvCOP1 and LvMYB1, with the latter being confirmed as a protein that limits the synthesis of anthocyanins. In light of these findings, we propose a model where LvCOP1, in the presence of light, can facilitate the ubiquitination and degradation of LvMYB1, thereby precisely regulating anthocyanin accumulation in lily. This study expands our understanding of COP1 functions, revealing its positive regulatory role in anthocyanin accumulation in lily.

中文翻译：

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LvCOP1 和 LvMYB1 之间的相互作用控制百合（Lilium 'Viviana'）中光诱导的花青素生物合成

组成型光形态发生蛋白 1 (COP1) 是一种泛素 E3 连接酶，已被确定为在无光情况下参与植物发育的关键阻遏蛋白。然而，它在百合（“Viviana”）中的具体作用仍然很大程度上未知。通过对百合的转录组分析，发现了一个对百合色素沉着有积极影响的基因（）。进化分析表明 是 的直系同源物，随后指定为 。 LvCOP1的启动子区富含多个光响应顺式作用元件，其表达受光调控，表明LvCOP1在响应光信号中发挥着至关重要的作用。此外，过度表达会提高百合花瓣中花青素的含量。这种增强是通过提高花青素生物合成中涉及的关键基因的表达水平来实现的，包括 、 和 。相反，沉默会产生相反的效果。此外，酵母文库筛选证明了 LvCOP1 和 LvMYB1 之间的相互作用，后者被确认为限制花青素合成的蛋白质。根据这些发现，我们提出了一个模型，其中 LvCOP1 在光存在下可以促进 LvMYB1 的泛素化和降解，从而精确调节百合中花青素的积累。这项研究扩展了我们对 COP1 功能的理解，揭示了其在百合花青素积累中的积极调节作用。