The cacao region in the state of Bahia, Brazil, has large areas of coastal plain where cacao plants are not grown as a result of chemical and physical limitations of the soil. The present study aimed to evaluate the proteomic profiles and identify differentially accumulated proteins (DAP) in roots of young plants of CCN 51 cacao genotype, grown in coastal plain compacted soil, with location and phosphorus (P) limitation. A cohesive yellow oxisol was placed in polyvinyl chloride (PVC) tubes, with dimensions of 0.24 m (height) x 0.1 m (internal diameter). The tubes were subdivided into three rings of 0.08 m height connected with tape. In the upper and lower rings soil density was maintained at 1 Mg m. In the lower ring, two levels of soil P (low - 200 mg m soil and ideal - 400 mg m soil) were evaluated. In the middle ring five soil densities (1; 1.3; 1.4, 1.5 and 1.7 mg m) were maintained. From proteomic analysis of the roots, 46 proteins were identified exclusively in the roots of the control plants, 47 proteins in the roots of the plants grown in the compacted soil (density of 1.7 Mg m) and with low P dose. Of these 9 proteins were classified as DAP and 38 as exclusive. In contrast, the roots of plants grown in compacted soil and with ideal P dose presented 99 proteins, of which 10 were DAP and 89 were exclusive. There was an increase in protein expression in roots grown in compacted soil, associated with the location and P ideal dose, whose proteins are involved in the metabolism of organic compounds, stress responses, biosynthetic processes and oxidation processes. Furthermore, most of the proteins identified in soil compaction treatments belonged to the group of proteins involved in metabolism of organic compounds. Proteins from this group, with greater expression, are related to the degradation of energy reserves during mechanical stress possibly caused by soil compaction. The difference in protein abundance, in each biological process, has demonstrated that the roots of young plants of CCN 51 cacao genotype have different response mechanisms to mechanical stress. These changes in the proteomic profile promoted the maintenance of cellular homeostasis and root system growth during stress processes in young cacao plants.

中文翻译：

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沿海平原压实土壤中生长的幼年可可植物根部的蛋白质组谱，具有位置和磷限制

巴西巴伊亚州的可可地区有大片沿海平原，由于土壤的化学和物理限制，这些地区无法种植可可植物。本研究旨在评估 CCN 51 可可基因型幼苗根部的蛋白质组谱并鉴定差异积累蛋白 (DAP)，这些幼苗生长在沿海平原压实土壤中，且受位置和磷 (P) 限制。将粘性黄色奥西索溶胶置于尺寸为 0.24 m（高度）x 0.1 m（内径）的聚氯乙烯 (PVC) 管中。将管子分成三个 0.08 m 高的环，用胶带连接。上环和下环土壤密度保持在1 Mg m。在下环中，评估了两个水平的土壤 P（低 - 200 mg m 土壤和理想 - 400 mg m 土壤）。中环维持五种土壤密度（1、1.3、1.4、1.5 和 1.7 mg m）。通过对根部的蛋白质组学分析，仅在对照植物的根部中鉴定出 46 种蛋白质，在压实土壤（密度为 1.7 Mg m）和低磷剂量下生长的植物的根部中鉴定出 47 种蛋白质。其中 9 种蛋白质被归类为 DAP，38 种被归类为专有蛋白质。相比之下，在压实土壤和理想磷剂量下生长的植物根部呈现出 99 种蛋白质，其中 10 种是 DAP，89 种是专有的。在压实土壤中生长的根部蛋白质表达增加，与位置和磷理想剂量有关，其蛋白质参与有机化合物的代谢、应激反应、生物合成过程和氧化过程。此外，在土壤压实处理中鉴定出的大多数蛋白质属于参与有机化合物代谢的蛋白质组。该组蛋白的表达量较高，与可能由土壤压实引起的机械应力期间能量储备的退化有关。每个生物过程中蛋白质丰度的差异表明，CCN 51 可可基因型幼苗的根部对机械胁迫具有不同的响应机制。蛋白质组谱的这些变化促进了年轻可可植物在胁迫过程中细胞稳态和根系生长的维持。