This is the first study to analyze the anti-inflammatory and antinociceptive effect of withanicandrin, isolated from Datura Ferox leaves, and the possible mechanism of action involved in adult zebrafish (ZFa). To this end, the animals were treated intraperitoneally (i.p.) with withanicandrin (4; 20 and 40 mg/kg; 20 μL) and subjected to locomotor activity and acute toxicity. Nociception tests were also carried out with chemical agents, in addition to tests to evaluate inflammatory processes induced by κ-Carrageenan 1.5% and a Molecular Docking study. As a result, withanicandrin reduced nociceptive behavior by capsaicin at a dose of 40 mg/kg and by acid saline at doses of 4 and 40 mg/kg, through neuromodulation of TRPV1 channels and ASICs, identified through blocking the antinociceptive effect of withanicandrin by the antagonists capsazepine and naloxone. Furthermore, withanicandrin caused an anti-inflammatory effect through the reduction of abdominal edema, absence of leukocyte infiltrate in the liver tissue and reduction of ROS in thel liver tissue and presented better affinity energy compared to control morphine (TRPV1) and ibuprofen (COX-1 and COX-2).

中文翻译：

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从 Datura Ferox 中分离出的 Withanicandrin 通过调节成年斑马鱼的 ASICS 和 TRPS 通道以及抗炎作用来促进镇痛

这是第一项分析从曼陀罗叶中分离出的醉茄甙的抗炎和抗伤害作用的研究，以及对成年斑马鱼（ZFa）可能的作用机制。为此，对动物进行腹膜内(ip)处理withanicandrin (4；20和40mg/kg；20μL)并进行运动活动和急性毒性。除了评估 κ-卡拉胶 1.5% 诱导的炎症过程的测试和分子对接研究之外，还使用化学试剂进行了伤害感受测试。结果，通过 TRPV1 通道和 ASIC 的神经调节，醉茄甙减少了剂量为 40 mg/kg 的辣椒素和剂量为 4 和 40 mg/kg 的盐水的伤害感受行为，这是通过阻断醉茄甙的抗伤害作用来确定的。拮抗剂辣椒西平和纳洛酮。此外，与对照吗啡（TRPV1）和布洛芬（COX-1）相比，醉茄甙通过减少腹部水肿、消除肝组织中的白细胞浸润以及减少肝组织中的ROS而产生抗炎作用，并且呈现出更好的亲和能。和 COX-2)。