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Comparative morphoanatomic and histochemical characterization of Cucurbita pepo L. specimens
Flora ( IF 1.9 ) Pub Date : 2024-04-09 , DOI: 10.1016/j.flora.2024.152510
Herlayne Carolayne Caetano da Silva , Cledson dos Santos Magalhães , Karina Perrelli Randau

is popularly known as pumpkin or zucchini. Compared to the others of the genus, it presents the greatest genetic variability. Thus, given its nutritional and medicinal benefits, it results in many widely cultivated and economically important specimens. Due to this, differentiation between specimens becomes necessary, mainly pumpkin and zucchini which have different characteristics, however they receive the same scientific nomenclature (). It is noteworthy that the anatomical knowledge of the specimens helps with taxonomy. Furthermore, due to the economic value, when it comes to vegetative propagation, the identification of structural aspects is important for the success of propagation, which depends on the regeneration of plant tissues. This study aimed to characterize and differentiate two specimens of through morphoanatomy and histochemistry. For anatomical study, usual methods in plant anatomy were used to prepare and analyze, under an optical microscope, semi-permanent slides containing transverse sections of the roots, stems, leaves, and paradermic sections of the leaf blades of specimens. Histochemical tests were performed on cross-sections of the specimens' leaf blades to locate chemical compounds. The optical microscopic evaluation enabled the anatomical characterization, revealing distinct characters among the specimens. For example, the zucchini shows crystals in the root. Meanwhile, the pumpkin shows sclerenchymatic pericycle in the stem and secretory ducts in the stem and leaves. Histochemical techniques showed the presence of different compounds, differentiating in the presence of starch in the zucchini and triterpenes and steroids in the pumpkin. Correct characterization provides important information for quality control of the plant drug and taxonomic differentiation of the specimens since diagnostic characters vary.

中文翻译:

Cucurbita pepo L. 标本的比较形态解剖学和组织化学特征

俗称南瓜或西葫芦。与该属的其他物种相比,它呈现出最大的遗传变异性。因此,鉴于其营养和药用价值,它产生了许多广泛种植且具有重要经济意义的标本。因此,有必要区分标本,主要是南瓜和西葫芦,它们具有不同的特征,但它们具有相同的科学命名法()。值得注意的是,标本的解剖学知识有助于分类学。此外,由于经济价值,当涉及无性繁殖时,结构方面的识别对于繁殖的成功非常重要,这取决于植物组织的再生。本研究旨在通过形态解剖学和组织化学来表征和区分两个标本。对于解剖学研究,使用植物解剖学中的常用方法在光学显微镜下制备和分析半永久性载玻片,其中包含标本的根、茎、叶的横切面和叶片的皮下切片。对样本叶片的横截面进行组织化学测试,以定位化学成分。光学显微镜评估能够进行解剖表征,揭示标本之间的独特特征。例如,西葫芦的根部有晶体。同时,南瓜在茎中显示出厚壁的中柱鞘,在茎和叶中显示出分泌管。组织化学技术显示存在不同的化合物,区分西葫芦中的淀粉和南瓜中的三萜和类固醇。由于诊断特征各不相同,正确的表征为植物药物的质量控制和标本的分类学区分提供了重要信息。
更新日期:2024-04-09
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