This study was conducted to generate and investigate the structural characteristics and antioxidant properties of a nano-composite film produced from Vicia ervilia protein (VEPI)—Launaea acanthodes gum (LAG). The film containing Silybum marianum extract microcapsules (SMEM) at varying volumes (0, 2.2, 7.5, 12.8, 15 v/v) along with different concentrations of graphene oxide nanoparticles (GONPs) (0, 0.6, 2.0, 3.4, 4.0 w/v). The thickness, solubility, and moisture content of the film samples increased after the integration of SMEM and GONPs (P < 0.05). Fourier-transform infrared analysis of the film sample based on VEPI/LAG/SMEM/GONPs showed proper interactions, including electrostatic interaction between these materials. X-ray diffraction analysis revealed a lower degree of crystallinity in the nano-composite film by adding GONPs and SMEM. The Differential scanning calorimetry analysis conducted on the composite and optimized films, integrating SMEM and GONPs, revealed an elevation in both the glass transition temperature and the thermal degradation temperature compared to the blank sample. The results obtained from Field emission scanning electron microscopy demonstrated that the type and quantity of variables significantly influenced the surface morphology of the films, leading to the formation of cracks and pores in the film. Furthermore, the findings regarding antimicrobial activity demonstrated that the nanocomposite film, incorporating SMEM and GONPs, exhibited antimicrobial properties against E. coli and S. aureus bacteria. According to our result VEPI/LAG/SMEM/GONPs nanocomposite film possess potential to be used as environment friendly packaging films to improve shelf life of food.

本研究的目的是生成并研究由蚕豆蛋白 (VEPI) —棘皮树胶 (LAG)生产的纳米复合膜的结构特征和抗氧化性能。该膜含有不同体积（0、2.2、7.5、12.8、15 v/v）的水飞蓟提取物微胶囊（SMEM）以及不同浓度的氧化石墨烯纳米粒子（GONP）（0、0.6、2.0、3.4、4.0 w/五）。 SMEM和GONPs整合后薄膜样品的厚度、溶解度和水分含量均有所增加（P  < 0.05）。基于 VEPI/LAG/SMEM/GONP 的薄膜样品的傅里叶变换红外分析显示了适当的相互作用，包括这些材料之间的静电相互作用。 X 射线衍射分析表明，添加 GONP 和 SMEM 的纳米复合薄膜的结晶度较低。对复合薄膜和优化薄膜进行的差示扫描量热分析（整合了 SMEM 和 GONP）显示，与空白样品相比，玻璃化转变温度和热降解温度均有所提高。场发射扫描电子显微镜的结果表明，变量的类型和数量显着影响薄膜的表面形貌，导致薄膜中裂纹和孔隙的形成。此外，抗菌活性的研究结果表明，含有 SMEM 和 GONP 的纳米复合膜对大肠杆菌和金黄色葡萄球菌具有抗菌特性。根据我们的研究结果，VEPI/LAG/SMEM/GONPs 纳米复合薄膜具有用作环境友好型包装薄膜的潜力，以提高食品的保质期。