Microbial interactions, particularly metabolic cross-feeding, play important roles in removing recalcitrant environmental pollutants; however, the underlying mechanisms involved in this process remain unclear. Thus, this study aimed to elucidate the mechanism by which metabolic cross-feeding occurs during synergistic dibenzofuran degradation between a highly efficient degrader, sp. strain p52, and a partner incapable of utilizing dibenzofuran. A bottom-up approach combined with pairwise coculturing was used to examine metabolic cross-feeding between strain p52 and sp. W06 or sp. D10. Pairwise coculture not only promoted bacterial pair growth but also facilitated dibenzofuran degradation. Specifically, strain p52, acting as a donor, released dibenzofuran metabolic intermediates, including salicylic acid and gentisic acid, for utilization and growth, respectively, by the partner strains W06 and D10. Both salicylic acid and gentisic acid exhibited biotoxicity, and their accumulation inhibited dibenzofuran degradation. The transcriptional activity of the genes responsible for the catabolism of dibenzofuran and its metabolic intermediates was coordinately regulated in strain p52 and its cocultivated partners, thus achieving synergistic dibenzofuran degradation. This study provides insights into microbial metabolic cross-feeding during recalcitrant environmental pollutant removal.

中文翻译：

---

主管降解者红球菌之间的代谢交叉喂养。菌株 p52 和二苯并呋喃分解代谢过程中的无能伙伴：了解主导作用和支持作用

微生物相互作用，特别是代谢交叉喂养，在去除顽固的环境污染物方面发挥着重要作用；然而，这一过程涉及的潜在机制仍不清楚。因此，本研究旨在阐明高效降解剂 sp. 之间协同二苯并呋喃降解过程中发生代谢交叉喂养的机制。菌株 p52 和不能利用二苯并呋喃的伙伴。采用自下而上的方法与成对共培养相结合来检查菌株 p52 和 sp. 之间的代谢交叉喂养。 W06 或 sp。 D10。配对共培养不仅促进细菌对生长，而且促进二苯并呋喃降解。具体来说，菌株p52作为供体，释放二苯并呋喃代谢中间体，包括水杨酸和龙胆酸，分别供伙伴菌株W06和D10利用和生长。水杨酸和龙胆酸均表现出生物毒性，它们的积累抑制二苯并呋喃的降解。在菌株 p52 及其共培养伙伴中，负责二苯并呋喃及其代谢中间体分解代谢的基因的转录活性受到协调调节，从而实现二苯并呋喃的协同降解。这项研究提供了对顽固环境污染物去除过程中微生物代谢交叉喂养的见解。