Microbial herbicide degradation is an efficient bioremediation method. In this study, a strain of , LM01, which efficiently degrades atrazine and nicosulfuron, was isolated from a corn field using a direct isolation method. The degradation effects of the identified strain on two herbicides were investigated and optimized using an artificial neural network. The maximum degradation rates of LM01 were 58.09 % and 42.97 % for atrazine and nicosulfuron, respectively. The degradation rate of atrazine in the soil reached 67.94 % when the concentration was 10 CFU/g after 5 d and was less effective than that of nicosulfuron. Whole genome sequencing of strain LM01 helped elucidate the possible degradation pathways of atrazine and nicosulfuron. The protein sequences of strain LM01 were aligned with the sequences of the degraded proteins of the two herbicides by using the National Center for Biotechnology Information platform. The sequence (GE005358, GE001556, GE004212, GE005218, GE004846, GE002487) with the highest query cover was retained and docked with the small-molecule ligands of the herbicides. The results revealed a binding energy of − 6.23 kcal/mol between GE005358 and the atrazine ligand and − 6.66 kcal/mol between GE002487 and the nicosulfuron ligand.

中文翻译：

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黑链霉菌 LM01 对莠去津和烟嘧磺隆的生物降解：性能、降解途径和可能涉及的基因

微生物除草剂降解是一种有效的生物修复方法。在本研究中，使用直接分离方法从玉米田中分离出一株 LM01，它能有效降解莠去津和烟嘧磺隆。使用人工神经网络研究并优化了已鉴定菌株对两种除草剂的降解效果。 LM01对莠去津和烟嘧磺隆的最大降解率分别为58.09%和42.97%。莠去津浓度为10 CFU/g，5 d后土壤中降解率达到67.94%，效果不及烟嘧磺隆。菌株 LM01 的全基因组测序有助于阐明莠去津和烟嘧磺隆可能的降解途径。利用国家生物技术信息中心平台，将菌株LM01的蛋白序列与两种除草剂的降解蛋白序列进行比对。保留具有最高查询覆盖度的序列（GE005358、GE001556、GE004212、GE005218、GE004846、GE002487）并与除草剂的小分子配体对接。结果显示，GE005358 和莠去津配体之间的结合能为 − 6.23 kcal/mol，GE002487 和烟嘧磺隆配体之间的结合能为 − 6.66 kcal/mol。