A novel fluorogenic alkyl diazirine photocrosslinker bearing an o-hydroxycinnamate moiety has been developed for identification of the targets of bioactive molecules. The o-hydroxycinnamate moiety can be converted to the corresponding 7-hydroxycoumarin derivative, which should be created on the interacting site within the photocaptured target protein. The label yield and fluorescence intensity have been immensely improved in comparison with our previous aromatic crosslinkers to facilitate target identification in small quantities.

中文翻译：

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使用带有肉桂酸酯部分的烷基二氮丙啶光交联剂对目标蛋白进行荧光标记

一种带有邻羟基肉桂酸酯部分的新型荧光烷基二氮丙啶光交联剂已被开发用于识别生物活性分子的靶标。邻羟基肉桂酸酯部分可以转化为相应的 7-羟基香豆素衍生物，该衍生物应该在光捕获的靶蛋白内的相互作用位点上产生。与我们之前的芳香族交联剂相比，标记产量和荧光强度得到了极大的提高，以方便小批量的目标识别。