To investigate the potential changes of glucose metabolism and glucose transporter protein (GLUT) in the visual cortex of formally deprived amblyopic rats, as well as the effects of enriched environments on the levels of nerve conduction and glucose metabolism in the visual cortex of amblyopic rats. 36 rats were randomly divided into three groups: CON + SE (n = 12), MD + SE (n = 12) and MD + EE (n = 12). The right eyelids of both MD + SE and MD + EE groups were sutured. After successful modelling, the MD + EE group was maintained in an enriched environment, and the other two groups were kept in the same environment. Pattern visual evoked potentials (PVEP) was used to confirm models' effect, glucose metabolism was analyzed by Micro-PET/CT (F-FDG), and the protein as well as mRNA expression levels of GLUT were detected by Western Blot and quantitative RT-PCR (quantitative Reverse Transcription-Polymerase Chain Reaction) analyses, site of GLUT expression by immunofluorescence (IF). After suture modelling, both the MD + EE and MD + SE groups objective visual nerve conduction function decreased, the glucose metabolism in the visual cortex was markedly lower. After the enriched environment intervention, it recovered in the MD + EE group. The expression levels of GLUT1 and GLUT3 were increased in the MD + EE group in comparison with the MD + SE group. GLUT1 was primarily expressed on astrocytes and endothelial cells, but GLUT3 was mainly expressed on neurons. Enrichment of the environment exhibited a therapeutic effect on amblyopia, which could be related to the enhancement of glucose metabolism and GLUT expression in the visual cortex.

中文翻译：

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环境富集对弱视大鼠视觉皮层GLUT表达的影响

探讨正式剥夺弱视大鼠视皮层葡萄糖代谢和葡萄糖转运蛋白（GLUT）的潜在变化，以及丰富环境对弱视大鼠视皮层神经传导和葡萄糖代谢水平的影响。 36只大鼠随机分为三组：CON + SE（n = 12）、MD + SE（n = 12）和MD + EE（n = 12）。 MD+SE组和MD+EE组均缝合右眼睑。建模成功后，MD+EE组维持在丰富的环境中，其他两组维持在相同的环境中。采用模式视觉诱发电位（PVEP）验证模型效果，Micro-PET/CT（F-FDG）分析糖代谢，Western Blot和定量RT检测GLUT蛋白及mRNA表达水平-PCR（定量逆转录聚合酶链反应）分析，通过免疫荧光 (IF) 检测 GLUT 表达位点。缝合造模后，MD+EE组和MD+SE组客观视觉神经传导功能均下降，视觉皮层葡萄糖代谢明显降低。 MD+EE组经过丰富环境干预后恢复。与MD+SE组相比，MD+EE组中GLUT1和GLUT3的表达水平增加。 GLUT1主要表达于星形胶质细胞和内皮细胞，而GLUT3主要表达于神经元。丰富环境对弱视具有治疗作用，这可能与视觉皮层葡萄糖代谢和GLUT表达的增强有关。