Electroporation is an effective technique for genetic manipulation of cells, both and . electroporation (IUE) is a special case, which represents a fine application of this technique to genetically modify specific tissues of embryos during prenatal development. Commercially available electroporators are expensive and not fully customizable. We have designed and produced an inexpensive, open-design, and customizable electroporator optimized for safe IUE. We introduce . We used off-the-shelf electrical parts, a single-board microcontroller, and a cheap data logger to build an open-design electroporator. We included a safety circuit to limit the applied electrical current to protect the embryos. We added full documentation, design files, and assembly instructions. output is on par with commercially available devices. Furthermore, the adjustable current limiter protects both the embryos and the uterus from overcurrent damage. A built-in data acquisition module provides real-time visualization and recordings of the actual voltage/current pulses applied to each embryo. Function of has been demonstrated by inducing focal cortical dysplasia in mice. The simple and fully open design enables quick and cheap construction of the device and facilitates further customization. The features of can accelerate the IUE technique implementation in any laboratory and speed up its learning curve.

中文翻译：

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NeuroPorator：一种开源限流电穿孔器，用于安全的子宫内基因转移

电穿孔是一种有效的细胞遗传操作技术。电穿孔（IUE）是一个特例，它代表了该技术在产前发育过程中对胚胎特定组织进行基因改造的良好应用。市售电穿孔器价格昂贵且不能完全定制。我们设计并生产了一种廉价、开放式设计、可定制的电穿孔器，针对安全 IUE 进行了优化。我们介绍。我们使用现成的电气部件、单板微控制器和廉价的数据记录器来构建开放式设计的电穿孔器。我们包括一个安全电路来限制施加的电流以保护胚胎。我们添加了完整的文档、设计文件和组装说明。输出与市售设备相当。此外，可调节电流限制器可以保护胚胎和子宫免受过电流损坏。内置数据采集模块提供施加到每个胚胎的实际电压/电流脉冲的实时可视化和记录。的功能已通过诱导小鼠局灶性皮质发育不良得到证实。简单且完全开放的设计使设备能够快速且廉价地构建，并有利于进一步定制。的特点可以加速IUE技术在任何实验室的实施并加快其学习曲线。